Gelboin H V, Huberman E, Sachs L
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1188-94. doi: 10.1073/pnas.64.4.1188.
Aryl hydrocarbon hydroxylase, an inducible microsomal enzyme system, has been measured in cultures of normal and transformed hamster, mouse, and human cells. In order to determine the highest level of enzyme, the cells were induced by pretreatment with benz(a)anthracene. A correlation was found between the level of enzyme activity and the susceptibility of the cells to the cytotoxicity produced after treatment with benzopyrene. The results indicate that aryl hydrocarbon hydroxylase is the enzyme system responsible for cell susceptibility to the cytotoxic effect of benzopyrene and the toxic effect of benzopyrene is due to its enzymatic conversion to a cytotoxic metabolite. 3-Hydroxybenzopyrene, one of the products of the enzymatic hydroxylation of benzopyrene, was found to be cytotoxic to cells that were either susceptible or resistant to the cytotoxic effect of benzopyrene.
芳烃羟化酶是一种可诱导的微粒体酶系统，已在正常及转化的仓鼠、小鼠和人类细胞培养物中进行了测定。为了确定酶的最高水平，细胞先用苯并(a)蒽预处理进行诱导。发现酶活性水平与细胞对苯并芘处理后产生的细胞毒性的敏感性之间存在相关性。结果表明，芳烃羟化酶是负责细胞对苯并芘细胞毒性作用敏感性的酶系统，苯并芘的毒性作用是由于其酶促转化为细胞毒性代谢物。苯并芘酶促羟化产物之一的3-羟基苯并芘，被发现对苯并芘细胞毒性作用敏感或抗性的细胞具有细胞毒性。
