Farrah S R, Goyal S M, Gerba C P, Conklin R H, Wallis C, Melnick J L, DuPont H L
Intervirology. 1978;9(1):56-9. doi: 10.1159/000148921.
Organic compounds in cell culture harvests known as membrane-coating components (MCC) prevent virus adsorption to membrane filters. Blending cell culture harvests with fluorocarbon removed the MCC and permitted adsorption of virus in acidified harvests to epoxy-fiberglass filters. Subsequent elution with high pH buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100-fold.
细胞培养收获物中的有机化合物，即膜包被成分（MCC），可阻止病毒吸附到膜过滤器上。将细胞培养收获物与碳氟化合物混合可去除MCC，并使酸化收获物中的病毒吸附到环氧玻璃纤维过滤器上。随后用高pH缓冲液洗脱，可回收超过90%的病毒，浓度可达100倍。
