一种使用膜滤器从细胞培养收获物中浓缩肠道病毒和轮状病毒的简单方法。
A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters.
作者信息
Farrah S R, Goyal S M, Gerba C P, Conklin R H, Wallis C, Melnick J L, DuPont H L
出版信息
Intervirology. 1978;9(1):56-9. doi: 10.1159/000148921.
PMID:202572
Abstract
Organic compounds in cell culture harvests known as membrane-coating components (MCC) prevent virus adsorption to membrane filters. Blending cell culture harvests with fluorocarbon removed the MCC and permitted adsorption of virus in acidified harvests to epoxy-fiberglass filters. Subsequent elution with high pH buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100-fold.
摘要
细胞培养收获物中的有机化合物，即膜包被成分（MCC），可阻止病毒吸附到膜过滤器上。将细胞培养收获物与碳氟化合物混合可去除MCC，并使酸化收获物中的病毒吸附到环氧玻璃纤维过滤器上。随后用高pH缓冲液洗脱，可回收超过90%的病毒，浓度可达100倍。
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