血清中庆大霉素的荧光分光光度测定法。

Spectrofluorometric assay of gentamicin in serum.

作者信息

Habbal Z M

出版信息

Clin Chim Acta. 1979 Jul 16;95(2):301-9. doi: 10.1016/0009-8981(79)90372-3.

Abstract

A spectrofluorometric method has been developed for the assay of gentamicin in serum. The fluorogen of this reaction is the Zn2+ chelate of N-pyridoxylidene gentamicin. The ratio of gentamicin to Zin is 1.0. Interfering compounds in serum are removed by chromatography on Bio-Rex 70. Recovery of added gentamicin is between 90 and 100%. Determination of gentamicin levels in patient sera correlate well with those done by bioassay. This assay is relatively fast (about 1 h), and requires 1.0 ml of serum. It also can be applied to the determination of other deoxystreptamine antibiotics such as kanamycin and tobramycin.

摘要

已开发出一种用于测定血清中庆大霉素的荧光分光光度法。该反应的荧光原是N-吡啶亚甲基庆大霉素的Zn2+螯合物。庆大霉素与锌的比例为1.0。血清中的干扰化合物通过在Bio-Rex 70上进行色谱分离去除。添加的庆大霉素回收率在90%至100%之间。患者血清中庆大霉素水平的测定结果与生物测定法的结果相关性良好。该测定相对较快（约1小时），需要1.0毫升血清。它也可用于测定其他脱氧链霉胺抗生素，如卡那霉素和妥布霉素。