Suppr超能文献

新城疫病毒病毒粒子中的核糖核酸聚合酶:与水疱性口炎病毒聚合酶的比较。

Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase.

作者信息

Huang A S, Baltimore D, Bratt M A

出版信息

J Virol. 1971 Mar;7(3):389-94. doi: 10.1128/JVI.7.3.389-394.1971.

Abstract

The virions of Newcastle disease virus (NDV) contained an enzyme that catalyzed the incorporation of ribonucleotides into ribonucleic acid (RNA). Optimal conditions for this polymerase activity were identical to the conditions for the vesicular stomatitis virus (VSV) polymerase, and both enzymes were active for longer times at 32 C than at 37 C. However, the specific activity of the NDV polymerase was less than 3% that of the VSV polymerase. Product RNA species from the NDV and VSV polymerase reactions annealed specifically to the homologous virion RNA species. Transcriptive intermediates containing product RNA attached to the respective virion RNA could be identified in both systems.

摘要

新城疫病毒(NDV)的病毒粒子含有一种能催化核糖核苷酸掺入核糖核酸(RNA)的酶。这种聚合酶活性的最佳条件与水泡性口炎病毒(VSV)聚合酶的条件相同,并且两种酶在32℃下比在37℃下具有更长时间的活性。然而,NDV聚合酶的比活性不到VSV聚合酶的3%。NDV和VSV聚合酶反应产生的RNA产物能与同源病毒粒子RNA特异性退火。在两个系统中都能鉴定出含有附着在各自病毒粒子RNA上的产物RNA的转录中间体。

相似文献

引用本文的文献

9
RNA polymerase in mumps virion.腮腺炎病毒粒子中的RNA聚合酶。
J Virol. 1974 Jul;14(1):183-6. doi: 10.1128/JVI.14.1.183-186.1974.

本文引用的文献

6
Isolation of the nucleic acid of Newcastle disease virus (NDV).新城疫病毒(NDV)核酸的分离
Proc Natl Acad Sci U S A. 1965 Sep;54(3):794-800. doi: 10.1073/pnas.54.3.794.

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验