Huang A S, Baltimore D, Bratt M A
J Virol. 1971 Mar;7(3):389-94. doi: 10.1128/JVI.7.3.389-394.1971.
The virions of Newcastle disease virus (NDV) contained an enzyme that catalyzed the incorporation of ribonucleotides into ribonucleic acid (RNA). Optimal conditions for this polymerase activity were identical to the conditions for the vesicular stomatitis virus (VSV) polymerase, and both enzymes were active for longer times at 32 C than at 37 C. However, the specific activity of the NDV polymerase was less than 3% that of the VSV polymerase. Product RNA species from the NDV and VSV polymerase reactions annealed specifically to the homologous virion RNA species. Transcriptive intermediates containing product RNA attached to the respective virion RNA could be identified in both systems.
新城疫病毒(NDV)的病毒粒子含有一种能催化核糖核苷酸掺入核糖核酸(RNA)的酶。这种聚合酶活性的最佳条件与水泡性口炎病毒(VSV)聚合酶的条件相同,并且两种酶在32℃下比在37℃下具有更长时间的活性。然而,NDV聚合酶的比活性不到VSV聚合酶的3%。NDV和VSV聚合酶反应产生的RNA产物能与同源病毒粒子RNA特异性退火。在两个系统中都能鉴定出含有附着在各自病毒粒子RNA上的产物RNA的转录中间体。