水泡性口炎病毒转录酶的完全转录
Complete transcription by the transcriptase of vesicular stomatitis virus.
作者信息
Bishop D H
出版信息
J Virol. 1971 Apr;7(4):486-90. doi: 10.1128/JVI.7.4.486-490.1971.
Abstract
Vesicular stomatitis virus (VSV) transcriptase completely transcribes the VSV genome into messenger-size ribonucleic acid (RNA) species. The replicating complexes of template and product RNA, generated in vitro by VSV transcriptase, exhibit a mass parity between product and template molecules. Purified product RNA, after annealing to small amounts of viral RNA, rendered 92% of the viral RNA ribonuclease-resistant. It is calculated that, under optimal conditions, 60 min is required before the VSV genome is completely transcribed.
摘要
水泡性口炎病毒（VSV）转录酶可将VSV基因组完全转录为信使大小的核糖核酸（RNA）种类。由VSV转录酶在体外产生的模板RNA和产物RNA的复制复合物，在产物分子和模板分子之间表现出质量均等。纯化后的产物RNA与少量病毒RNA退火后，使92%的病毒RNA具有核糖核酸酶抗性。据计算，在最佳条件下，VSV基因组完全转录需要60分钟。
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