Ericsson R J, Buthala D A, Norland J F
Science. 1971 Jul 2;173(3991):54-5. doi: 10.1126/science.173.3991.54.
Fertilization occurs when rabbit ova are cultured in vitro with epididymal sperm to which Sendai virus is adsorbed. These sperm do not require capacitation in vivo in order to fertilize. Evidence for fertilization is penetration of sperm, the appearance of two polar bodies and pronuclei, and cleavage through the eight-blastomere stage. The viruses attach almost exclusively to the sperm acrosome, with resultant head-to-head agglutination of the sperm.
当兔卵子与吸附了仙台病毒的附睾精子在体外培养时，受精就会发生。这些精子在体内不需要获能就能受精。受精的证据包括精子穿透、两个极体和原核的出现以及卵裂至八细胞期。病毒几乎只附着在精子顶体上，导致精子头对头凝集。
