Karin M, Herschman H R
Science. 1979 Apr 13;204(4389):176-7. doi: 10.1126/science.432639.
HeLa cells in culture synthesize metallothionein. To investigate the effects of glucocorticoids on metallothionein synthesis we adapted HeLa cells to growth in a defined medium lacking hydrocortisone. In this defined medium, containing 1.5 x 10(-6)M Zn2+, dexamethasone (10(-6)M) caused a five- to sixfold increase in the synthesis of metallothionein. Progesterone (10(-5)M), a known antagonist of glucocorticoids, inhibited this response by 50 percent.
培养中的HeLa细胞能合成金属硫蛋白。为了研究糖皮质激素对金属硫蛋白合成的影响，我们使HeLa细胞适应在缺乏氢化可的松的限定培养基中生长。在这种含有1.5×10⁻⁶M Zn²⁺的限定培养基中，地塞米松（10⁻⁶M）使金属硫蛋白的合成增加了五到六倍。孕酮（10⁻⁵M），一种已知的糖皮质激素拮抗剂，抑制了50%的这种反应。
