地塞米松和锌诱导HeLa细胞中金属硫蛋白的特性研究。

Characterization of the metallothioneins induced in HeLa cells by dexamethasone and zinc.

作者信息

Karin M, Herschman H R

出版信息

Eur J Biochem. 1980 Jun;107(2):395-401. doi: 10.1111/j.1432-1033.1980.tb06042.x.

DOI:10.1111/j.1432-1033.1980.tb06042.x

Abstract

The metallothioneins induced in HeLa cells by dexamethasone and Zn2+ were labeled with [3H]cysteine and [35S]cysteine respectively. These labeled metallothioneins were compared to one another and to metallothioneins from human liver by (a) gel permeation chromatography on Sephadex G-75 columsn, (b) ion-exchange chromatograhy on DEAE-Sephadex A-25 columns, (c) electrophoresis on non-denaturing polyacrylamide gels, and (d) electrophoresis of reduced and alkylated proteins on dodecylsulfate/polyacrylamide gels. The dexamethasone-induced metallothioneins of HeLa cells, the Zn2+-induced metallothioneins of HeLa cells, and the metallothioneins of human liver were indistinguishable by these four criteria.

摘要

分别用[³H]半胱氨酸和[³⁵S]半胱氨酸标记地塞米松和Zn²⁺在HeLa细胞中诱导产生的金属硫蛋白。通过以下方法将这些标记的金属硫蛋白相互比较，并与人肝脏中的金属硫蛋白进行比较：(a)在Sephadex G - 75柱上进行凝胶渗透色谱法；(b)在DEAE - Sephadex A - 25柱上进行离子交换色谱法；(c)在非变性聚丙烯酰胺凝胶上进行电泳；(d)在十二烷基硫酸盐/聚丙烯酰胺凝胶上对还原和烷基化的蛋白质进行电泳。根据这四个标准，HeLa细胞中地塞米松诱导的金属硫蛋白、HeLa细胞中Zn²⁺诱导的金属硫蛋白以及人肝脏中的金属硫蛋白无法区分。