Hsie A W, Jones C, Puck T T
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1648-52. doi: 10.1073/pnas.68.7.1648.
The morphological conversion in vitro of Chinese hamster ovary cells to a fibroblast form by a relatively large amount of dibutyryl adenosine cyclic 3':5'-monophosphate, or by a combination of small amounts of this compound and testosterone, is attended by appearance of the following additional properties: acquisition of strict contact inhibition of growth; reorientation of the random growth pattern into one in which cells grow parallel to their long dimension; disappearance of the randomly distributed, knob-like, pseudopodal structures around the cell periphery; induction of collagen synthesis; and decrease in the ability to be agglutinated and rounded up by plant agglutinins and specific cell antibodies. The changes in these characteristics are consistent with the conversion from a malignant to a normal fibroblastic state. This conversion is under genetic control, as demonstrated by the production of specific mutants with altered characteristics. The response to testosterone is specific since steroids like estradiol and hydrocortisone are inactive, and others have limited activity. Some prostaglandins are equal in activity to testosterone and 5alpha-dihydrotestosterone. This system appears useful in study of the regulation of phenotypic expression in mammalian cells.
用相对大量的二丁酰腺苷环3':5'-单磷酸，或用少量该化合物与睾酮的组合，可使中国仓鼠卵巢细胞在体外形态转化为成纤维细胞形式，同时还会出现以下其他特性：获得严格的生长接触抑制；将随机生长模式重新定向为细胞沿其长轴平行生长的模式；细胞周边随机分布的、瘤状伪足结构消失；诱导胶原蛋白合成；以及被植物凝集素和特异性细胞抗体凝集并变圆的能力下降。这些特性的变化与从恶性状态向正常成纤维细胞状态的转化一致。这种转化受遗传控制，如产生具有改变特性的特定突变体所证明。对睾酮的反应具有特异性，因为雌二醇和氢化可的松等类固醇无活性，而其他类固醇活性有限。一些前列腺素的活性与睾酮和5α-二氢睾酮相当。该系统似乎有助于研究哺乳动物细胞中表型表达的调控。
