培养的中国仓鼠卵巢细胞中高分子量膜蛋白种类的磷酸化：6 - N，2'- O - 二丁酰腺苷3':5'-环一磷酸加睾酮的作用
Phosphorylation of high-molecular-weight membrane protein species in Chinese-hamster ovary cells in culture: effect of 6-N,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate plus testosterone.
作者信息
Rieber M, Bacalao J
出版信息
Biochem J. 1973 Mar;132(3):641-4. doi: 10.1042/bj1320641.
Abstract
Growth of Chinese-hamster ovary cells in [(32)P]phosphate and [(3)H]leucine and subsequent assay of the plasma membranes reveals phosphorylation in two protein regions corresponding to molecular weights of 280000 and 195000. Culture in the presence of the 6-N,2-O'-dibutyryl derivative of cyclic AMP plus testosterone does not stimulate [(32)P]-phosphate incorporation, but determines a modification in the qualitative pattern of phosphorylation.
摘要
中国仓鼠卵巢细胞在[（32）P]磷酸盐和[（3）H]亮氨酸中生长，随后对质膜进行分析，结果显示在分子量分别为280000和195000的两个蛋白质区域存在磷酸化现象。在环磷酸腺苷的6-N，2-O'-二丁酰衍生物加睾酮存在的情况下进行培养，并不会刺激[（32）P] - 磷酸盐的掺入，但会导致磷酸化定性模式的改变。
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Phosphorylation of high-molecular-weight membrane protein species in Chinese-hamster ovary cells in culture: effect of 6-N,2'-O-dibutyryladenosine 3':5'-cyclic monophosphate plus testosterone.培养的中国仓鼠卵巢细胞中高分子量膜蛋白种类的磷酸化：6 - N，2'- O - 二丁酰腺苷3':5'-环一磷酸加睾酮的作用
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