Robinson W S
J Virol. 1971 Jul;8(1):81-6. doi: 10.1128/JVI.8.1.81-86.1971.
After dissociation of purified Sendai virus with the neutral detergent Nonidet P-40 and 2-mercaptoethanol, it catalyzed the incorporation of ribonucleoside triphosphates into an acid-insoluble product. The enzyme activity was associated with viral nucleocapsid as well as whole virions. The reaction product was ribonucleic acid (RNA) which annealed specifically with virion RNA. Sedimentation of the (3)H-RNA reaction product revealed two components, a 45S component with properties of double-stranded RNA and 4 to 6S component which appeared to be mostly single-stranded RNA.
用中性去污剂Nonidet P - 40和2 - 巯基乙醇解离纯化的仙台病毒后,它催化三磷酸核糖核苷掺入酸不溶性产物中。酶活性与病毒核衣壳以及完整病毒粒子相关。反应产物是核糖核酸(RNA),它与病毒粒子RNA特异性退火。(3)H - RNA反应产物的沉降显示出两个组分,一个具有双链RNA性质的45S组分和一个似乎主要是单链RNA的4至6S组分。