蛙病毒3对单链和双链RNA的降解作用
Degradation of single- and double-stranded RNA by frog virus 3.
作者信息
Palese P, Koch G
出版信息
Proc Natl Acad Sci U S A. 1972 Mar;69(3):698-701. doi: 10.1073/pnas.69.3.698.
Abstract
Purified preparations of frog virus 3 possess ribonuclease activities directed against single-and double-stranded RNA. Double-stranded RNAs isolated from purified reovirus type 3 and from HeLa cells infected with poliovirus and single-stranded poliovirus RNA from purified virus are readily degraded by incubation with frog virus 3. The mode of action of the nucleases is endonucleolytic. Under the assay conditions used for the viral enzyme, crude extracts of uninfected HeLa, L, and baby hamster kidney cells did not show enzyme activity against double-stranded RNA but exhibited activity against single-stranded RNA. The dependence of the viral nucleases on divalent cations for optimal activity and the inhibition of the cleavage of single-stranded RNA by 0.2 M NaCl suggests that the enzymes are either virus-coded or virus-induced.
摘要
纯化的青蛙病毒3制剂具有针对单链和双链RNA的核糖核酸酶活性。从纯化的3型呼肠孤病毒以及感染脊髓灰质炎病毒的HeLa细胞中分离出的双链RNA，和从纯化病毒中获得的单链脊髓灰质炎病毒RNA，在与青蛙病毒3一起温育时很容易被降解。这些核酸酶的作用方式是内切核酸酶。在用于检测病毒酶的条件下，未感染的HeLa、L和幼仓鼠肾细胞的粗提取物对双链RNA没有酶活性，但对单链RNA表现出活性。病毒核酸酶对二价阳离子具有最佳活性的依赖性，以及0.2M NaCl对单链RNA切割的抑制作用表明，这些酶要么是病毒编码的，要么是病毒诱导的。
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