口蹄疫病毒抗体的检测。II. 使用分级分离的牛抗血清提高“被动”血凝试验的特异性。

Detection of foot-and-mouth disease virus antibodies. II. Use of fractionated bovine antisera for improving the specificity of a "passive" hemagglutination test.

作者信息

Warrington R E, Kawakami Y

出版信息

Appl Microbiol. 1971 Jul;22(1):37-43. doi: 10.1128/am.22.1.37-43.1971.

DOI:10.1128/am.22.1.37-43.1971

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC377373/

Abstract

Because 7S immunoglobulin (Ig) G antibodies of low type specificity were present in mixtures with highly specific 19S IgM antibodies, many bovine antisera to foot-and-mouth disease virus (FMDV) type A(12), strain 119 cross-reacted with type O of FMDV and to some degree with type C in the passive hemagglutination (HA) test. After 19S IgM antibodies were separated by density gradient centrifugation or precipitated with 4% (w/v) polyethylene glycol, the antigen could be determined with "block" HA tests. Such tests used several antigen concentrations in the titration of each antiserum. Adding 4% (w/v) polyethylene glycol to the serum was especially convenient for rapid precipitation of 19S IgM antibodies for the test. Similar results were obtained with bovine 19S IgM antibodies to FMDV type O, subtype 1, strain Caseros and type C strain Rezende.

摘要

由于低型特异性的7S免疫球蛋白（Ig）G抗体与高特异性的19S IgM抗体混合存在，许多针对口蹄疫病毒（FMDV）A（12）型119株的牛抗血清在被动血凝试验（HA）中与FMDV O型发生交叉反应，并在一定程度上与C型发生交叉反应。通过密度梯度离心分离19S IgM抗体或用4%（w/v）聚乙二醇沉淀后，可用“阻断”HA试验测定抗原。此类试验在滴定每种抗血清时使用了几种抗原浓度。向血清中加入4%（w/v）聚乙二醇对于快速沉淀用于试验的19S IgM抗体特别方便。针对FMDV O型1亚型Caseros株和C型Rezende株的牛19S IgM抗体也获得了类似结果。

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引用本文的文献

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Appl Microbiol. 1972 Jan;23(1):98-103. doi: 10.1128/am.23.1.98-103.1972.

本文引用的文献

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The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera.蛋白质在经单宁酸处理的红细胞上的吸附以及随后抗蛋白质血清引起的血细胞凝集。

J Exp Med. 1951 Feb;93(2):107-20. doi: 10.1084/jem.93.2.107.

2

ANTIBODIES PRODUCED BY GUINEA PIGS INFECTED WITH FOOT-AND-MOUTH DISEASE VIRUS.感染口蹄疫病毒的豚鼠产生的抗体。

J Immunol. 1965 Jun;94:858-67.

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PRODUCTION AND PURIFICATION OF MILLIGRAM AMOUNTS OF FOOT-AND-MOUTH DISEASE VIRUS FROM BABY HAMSTER KIDNEY CELL CULTURES.从幼仓鼠肾细胞培养物中生产和纯化毫克量的口蹄疫病毒

Appl Microbiol. 1964 Jul;12(4):368-73. doi: 10.1128/am.12.4.368-373.1964.

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CHEMICAL PHYSICAL PROPERTIES OF VIRTUALLY PURE FOOT-AND-MOUTH DISEASE VIRUS.近乎纯口蹄疫病毒的化学物理特性

Am J Vet Res. 1964 Mar;25:333-42.

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Micromethods for the study of proteins and antibodies. II. Specific applications of hemagglutination and hemagglutination-inhibition reactions with tannic acid and protein-treated red blood cells.蛋白质和抗体研究的微量方法。II. 鞣酸和蛋白质处理红细胞的血凝及血凝抑制反应的具体应用。

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