Warrington R E, Kawakami Y
Appl Microbiol. 1972 Jan;23(1):98-103. doi: 10.1128/am.23.1.98-103.1972.
In addition to currently used serological tests for the occurrence of foot-and-mouth disease virus (FMDV), a specific "passive" hemagglutination-inhibition (HAI) test has been developed as a supplement. Serial twofold dilutions of antiserum (0.05 ml) were mixed with 0.05 ml of a constant concentration of FMDV. After incubating for 30 min at 37 C, agglutinating antibodies were determined by adding 0.1 ml of 2.5% virus-sensitized erythrocytes. The minimum concentration of antiserum required to agglutinate the erythrocytes defined the inhibition in the HAI test. Similar tests using different concentrations of virus to inhibit antibodies were carried out in parallel fashion. The relationship between the logarithm of the HAI titer and the concentration of inhibiting virus was nearly first order (P > 0.25). The slope was used as a measure of the relative specificities of the antigen-antibody interaction and was independent of concentration. The HAI test was type-, subtype-, strain-, and variant-specific with the viral antigens used. In particular, typing was performed directly on bovine antisera.
除了目前用于检测口蹄疫病毒（FMDV）感染情况的血清学检测方法外，还开发了一种特定的“被动”血凝抑制（HAI）检测方法作为补充。将抗血清（0.05 ml）进行连续两倍稀释，与0.05 ml恒定浓度的口蹄疫病毒混合。在37℃孵育30分钟后，通过加入0.1 ml 2.5%的病毒致敏红细胞来测定凝集抗体。使红细胞凝集所需的抗血清最低浓度确定了HAI检测中的抑制作用。以平行方式进行了使用不同浓度病毒抑制抗体的类似检测。HAI滴度的对数与抑制病毒浓度之间的关系近似为一级关系（P>0.25）。斜率用作抗原-抗体相互作用相对特异性的指标，且与浓度无关。使用所采用的病毒抗原时，HAI检测具有型、亚型、毒株和变异株特异性。特别是，直接对牛抗血清进行分型。
