Kerr I M, Martin E M
J Virol. 1971 Apr;7(4):438-47. doi: 10.1128/JVI.7.4.438-447.1971.
Ribonucleic acid (RNA) from encephalomyocarditis (EMC) virus stimulates the incorporation of amino acids into protein in cell-free protein-synthetic systems derived from Krebs mouse ascites tumor cells and chick embryo fibroblasts; the mouse system is the more responsive to the viral RNA. The greater part of this difference in activity can be ascribed to the cell sap, but the origin of the ribosomes also has a marked effect. The nature of the polypeptides formed in these cell-free systems was investigated by electrophoresis on polyacrylamide gels and by fingerprint analysis of tryptic digests. The same product in part appears to be synthesized in response to the EMC RNA in both systems. It was not detected if the EMC RNA was partly degraded (</=4S) or replaced by other species of RNA, including that from influenza virus. The results suggest that EMC RNA is partially translated in these systems to yield virus-specific polypeptides.
来自脑心肌炎（EMC）病毒的核糖核酸（RNA）可刺激氨基酸掺入源自克雷布斯小鼠腹水瘤细胞和鸡胚成纤维细胞的无细胞蛋白质合成系统中的蛋白质；小鼠系统对病毒RNA的反应更强。这种活性差异的大部分可归因于细胞液，但核糖体的来源也有显著影响。通过聚丙烯酰胺凝胶电泳和胰蛋白酶消化物的指纹分析研究了这些无细胞系统中形成的多肽的性质。在两个系统中，部分相同的产物似乎是响应EMC RNA而合成的。如果EMC RNA部分降解（≤4S）或被其他种类的RNA替代，包括来自流感病毒的RNA，则无法检测到该产物。结果表明，EMC RNA在这些系统中部分被翻译以产生病毒特异性多肽。
