发育过程中大鼠肝脏中的胸苷激酶
Thymidine kinase in rat liver during development.
作者信息
Klemperer H G, Haynes G R
出版信息
Biochem J. 1968 Jul;108(4):541-6. doi: 10.1042/bj1080541.
Abstract
- The activity of thymidine kinase in rat liver supernatant decreased with development to a value in the adult that was 1% of that in the 17-day foetus. 2. The foetal enzyme was more stable than the adult to gel filtration on Sephadex G-25 at 0 degrees . 3. The greater stability of the foetal enzyme to incubation at 45 degrees was attributable to the presence of higher concentrations of nucleotides in foetal liver supernatant. 4. The K(m) values for foetal and adult enzymes were approx. 2.5mum- and 2.1mum-thymidine respectively. 5. The foetal enzyme was more sensitive to inhibition by thymidine triphosphate. 6. The decline in enzyme activity during the neonatal period was correlated with a shift in the enzyme properties from the foetal to the adult type, and may reflect the decrease in the proportion of haemopoietic tissue in the liver.
摘要
- 大鼠肝脏上清液中胸苷激酶的活性随着发育而降低，在成年时的值仅为17日龄胎儿时的1%。2. 在0℃下，胎儿酶在Sephadex G - 25凝胶过滤中比成人酶更稳定。3. 胎儿酶在45℃孵育时具有更高的稳定性，这归因于胎儿肝脏上清液中核苷酸浓度较高。4. 胎儿和成人酶的K(m)值分别约为2.5μM和2.1μM胸苷。5. 胎儿酶对三磷酸胸苷的抑制更敏感。6. 新生儿期酶活性的下降与酶性质从胎儿型向成人型的转变相关，这可能反映了肝脏中造血组织比例的降低。
相似文献
1
Thymidine kinase in rat liver during development.发育过程中大鼠肝脏中的胸苷激酶
Biochem J. 1968 Jul;108(4):541-6. doi: 10.1042/bj1080541.
2
Purification and properties of thymidine kinase from regenerating rat liver.再生大鼠肝脏中胸苷激酶的纯化及性质
Biochim Biophys Acta. 1974 May 20;350(1):193-200. doi: 10.1016/0005-2744(74)90217-4.
3
Thymidine kinase activity in cardiac muscle during embryomic and postnatal development.胚胎期及出生后发育过程中心肌中的胸苷激酶活性。
Biochem J. 1974 Sep;142(3):685-90. doi: 10.1042/bj1420685.
4
[Some properties of cytoplasmic thymidine kinase and nucleoside phosphotransferase from rat liver].[大鼠肝脏细胞质胸苷激酶和核苷磷酸转移酶的一些特性]
Biokhimiia. 1977 Sep;42(9):1668-73.
5
Some properties of galactokinase in developing rat liver.发育中大鼠肝脏中半乳糖激酶的某些特性
Biochem J. 1968 Jun;108(2):169-75. doi: 10.1042/bj1080169.
6
The ontogeny of thymidine kinase in tissues of man and rat.人和大鼠组织中胸苷激酶的个体发生。
Pediatr Res. 1980 Dec;14(12):1304-10. doi: 10.1203/00006450-198012000-00006.
7
Uridine--cytidine kinase from foetal and adult rat liver. Purification and study of some properties.来自胎鼠和成年大鼠肝脏的尿苷 - 胞苷激酶。纯化及某些性质的研究。
Eur J Biochem. 1976 Oct 1;69(1):217-22. doi: 10.1111/j.1432-1033.1976.tb10876.x.
8
Properties of thymidine kinase partially purified from human fetal and adult tissue.从人胎儿和成人组织中部分纯化的胸苷激酶的性质。
J Biol Chem. 1972 Mar 25;247(6):1930-5.
9
Glutamate dehydrogenase, alanine aminotransferase, thymidine kinase, and arginase in fetal and adult human and rat liver.胎儿及成年人类和大鼠肝脏中的谷氨酸脱氢酶、丙氨酸转氨酶、胸苷激酶和精氨酸酶。
Pediatr Res. 1976 Dec;10(12):960-4. doi: 10.1203/00006450-197612000-00002.
10
Phenolsulfotransferase in the developing rat.发育中大鼠的酚磺基转移酶
Am J Clin Nutr. 1969 Aug;22(8):978-85. doi: 10.1093/ajcn/22.8.978.
引用本文的文献
1
Thymidine kinase 1 through the ages: a comprehensive review.胸腺嘧啶激酶1的历史沿革：一篇综述
Cell Biosci. 2020 Nov 27;10(1):138. doi: 10.1186/s13578-020-00493-1.
2
Intestinal epithelial stem/progenitor cells are controlled by mucosal afferent nerves.肠上皮干细胞/祖细胞受黏膜传入神经控制。
PLoS One. 2011 Feb 9;6(2):e16295. doi: 10.1371/journal.pone.0016295.
3
Reduced gastrointestinal toxicity following inhibition of the biliary excretion of irinotecan and its metabolites by probenecid in rats.丙磺舒抑制大鼠体内伊立替康及其代谢产物的胆汁排泄后，胃肠道毒性降低。
Pharm Res. 2002 Sep;19(9):1345-53. doi: 10.1023/a:1020358910490.
4
Biochemical consequences of 5-fluorouracil gastrointestinal toxicity in rats; effect of high-dose uridine.5-氟尿嘧啶对大鼠胃肠道毒性的生化后果；高剂量尿苷的影响。
Cancer Chemother Pharmacol. 1993;32(3):243-8. doi: 10.1007/BF00685843.
5
Elevated serum thymidine kinase activity in patients with acute viral hepatitis.急性病毒性肝炎患者血清胸苷激酶活性升高。
Gastroenterol Jpn. 1993 Feb;28(1):51-5. doi: 10.1007/BF02775003.
6
Effect of arabinosyl cytosine on the level of DNA polymerase and thymidine kinase activity in PHA-stimulated human tonsillar lymphocytes.阿糖胞苷对PHA刺激的人扁桃体淋巴细胞中DNA聚合酶水平和胸苷激酶活性的影响。
Mol Cell Biochem. 1982 Jan 16;42(1):37-44. doi: 10.1007/BF00223537.
7
Effect of a single high dose and repeated small doses of dianhydrogalactitol (DAG; NSC-132313) on rat intestinal mucosa.单次高剂量和重复小剂量双去水半乳糖醇（DAG；NSC-132313）对大鼠肠黏膜的影响。
Cancer Chemother Pharmacol. 1983;11(3):167-71. doi: 10.1007/BF00254198.
8
Stabilization of SV40 transformed human fibroblast cytoplasmic thymidine kinase by ATP.ATP对SV40转化的人成纤维细胞胞质胸苷激酶的稳定作用。
Mol Cell Biochem. 1981 Feb 26;35(1):59-64. doi: 10.1007/BF02358189.
9
Thymidine kinase activity in cardiac muscle during embryomic and postnatal development.胚胎期及出生后发育过程中心肌中的胸苷激酶活性。
Biochem J. 1974 Sep;142(3):685-90. doi: 10.1042/bj1420685.
10
Thymidine kinase of rat liver. Activation by phospholipase C and subcellular distribution in the liver.大鼠肝脏的胸苷激酶。磷脂酶C的激活作用及在肝脏中的亚细胞分布。
Biochem J. 1971 Apr;122(3):347-51. doi: 10.1042/bj1220347.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
DISTRIBUTION OF FORMS OF LACTIC DEHYDROGENASE WITHIN THE DEVELOPING RAT KIDNEY.发育中大鼠肾脏内乳酸脱氢酶各形式的分布
Dev Biol. 1963 Oct;8:151-64. doi: 10.1016/0012-1606(63)90039-3.
3
Feedback inhibition of thymodine kinase by thymodine triphosphate.三磷酸胸苷对胸苷激酶的反馈抑制作用。
J Biol Chem. 1963 Apr;238:1467-74.
4
5
Tryptophan peroxidase-oxidase, histidase, and transaminase activity in the liver of the developing rat.发育中大鼠肝脏中的色氨酸过氧化物酶-氧化酶、组氨酸酶和转氨酶活性
J Biol Chem. 1959 Feb;234(2):304-6.
6
Rates of turnover of deoxythymidine kinase and of its template RNA in regenerating and control liver.再生肝脏和对照肝脏中脱氧胸苷激酶及其模板RNA的周转率
Cancer Res. 1967 Mar;27(3):469-75.
7
Regulation of mammalian deoxyribonucleotide biosynthesis by nucleotides as activators and inhibitors.核苷酸作为激活剂和抑制剂对哺乳动物脱氧核糖核苷酸生物合成的调控。
J Biol Chem. 1966 Oct 25;241(20):4802-9.
8
Control of aspartate transcarbamylase activity in type 5 adenovirus-infected HeLa cells.5型腺病毒感染的HeLa细胞中天冬氨酸转氨甲酰酶活性的调控
J Bacteriol. 1964 May;87(5):1027-33. doi: 10.1128/jb.87.5.1027-1033.1964.
9
Template stability of some enzymes in rat liver and hepatoma.大鼠肝脏和肝癌中某些酶的模板稳定性
Proc Natl Acad Sci U S A. 1965 Sep;54(3):845-51. doi: 10.1073/pnas.54.3.845.
10
The molecular correlation concept of neoplasia.肿瘤形成的分子关联概念。
Adv Enzyme Regul. 1966;4:115-45. doi: 10.1016/0065-2571(66)90011-2.
Zotero 插件