Coffin J M, Temin H M
J Virol. 1971 May;7(5):625-34. doi: 10.1128/JVI.7.5.625-634.1971.
Labeled virions of Rous sarcoma virus (RSV) were disrupted with detergent and analyzed on equilibrium sucrose density gradients. A core fraction at a density of approximately 1.24 g/cc contained all of the (3)H-uridine label and about 30% of the (3)H-leucine label from the virions. Endogenous viral deoxyribonucleic acid (DNA) polymerase activity was only found in the same location. Additional ribonucleic acid (RNA)- and DNA-dependent DNA polymerase activities were found at the top of the gradients. RNA-dependent and DNA-dependent DNA polymerase activities were also found in RSV-converted chicken cells. Particles containing these activities were released from cells by detergent and were shown to contain viral RNA. These particles were analyzed on equilibrium sucrose density gradients and were found to have densities different from virion cores.
用去污剂破坏劳氏肉瘤病毒（RSV）的标记病毒粒子，并在平衡蔗糖密度梯度上进行分析。密度约为1.24 g/cc的核心组分含有来自病毒粒子的所有（3）H-尿苷标记和约30%的（3）H-亮氨酸标记。内源性病毒脱氧核糖核酸（DNA）聚合酶活性仅在同一位置被发现。在梯度顶部还发现了额外的核糖核酸（RNA）依赖性和DNA依赖性DNA聚合酶活性。在RSV转化的鸡细胞中也发现了RNA依赖性和DNA依赖性DNA聚合酶活性。含有这些活性的颗粒通过去污剂从细胞中释放出来，并显示含有病毒RNA。这些颗粒在平衡蔗糖密度梯度上进行分析，发现其密度与病毒粒子核心不同。
