2型单纯疱疹病毒补体结合抗原的制备
Preparation of type 2 herpes simplex virus complement-fixing antigen.
作者信息
Palmer E L, Martin M L, Warfield D T
出版信息
Appl Microbiol. 1971 Nov;22(5):925-7. doi: 10.1128/am.22.5.925-927.1971.
Abstract
Comparable complement-fixing antigens of type 1 and type 2 herpes simplex virus were produced by extraction of infected African green monkey cells with 0.85% NaCl which was buffered at pH 9.0 with 0.05 m glycine-NaOH. The optimal antigen dilutions were higher in titrations against hyperimmune animal sera than in titrations against human sera. Complement-fixing antibody to type 2 herpes antigen was detected in 5 of 17 sera from healthy humans.
摘要
通过用0.05m甘氨酸 - 氢氧化钠在pH 9.0缓冲的0.85%氯化钠提取感染的非洲绿猴细胞，制备了1型和2型单纯疱疹病毒的可比补体结合抗原。在针对超免疫动物血清的滴定中，最佳抗原稀释度高于针对人血清的滴定。在17份健康人血清中的5份中检测到了针对2型疱疹抗原的补体结合抗体。
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