磷脂在囊泡膜中的侧向扩散。
Lateral diffusion of phospholipids in a vesicle membrane.
作者信息
Kornberg R D, McConnell H M
出版信息
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2564-8. doi: 10.1073/pnas.68.10.2564.
Abstract
Nuclear-resonance spectra of phosphatidylcholine (PC) vesicles are broadened by spin-labeled PC. The broadening of the N-methyl proton line is proportional to spin-labeled PC concentration; the broadening by 1 molecule in 100 of spin-labeled PC is 9.7 Hz at 35 degrees C; the broadening is not due to collisions between vesicles, exchange of spin label between vesicles, or fusion of vesicles. These findings are proof of rapid diffusion in the plane of the PC bilayer (lateral diffusion). A further study of the broadening reveals the molecular frequency of the translation step for lateral diffusion to be much greater than 3 x 10(3) sec(-1) at 0 degrees C.
摘要
磷脂酰胆碱（PC）囊泡的核磁共振谱会因自旋标记的PC而变宽。N - 甲基质子线的变宽与自旋标记的PC浓度成正比；在35℃时，每100个自旋标记的PC分子中有1个导致的变宽为9.7赫兹；这种变宽不是由于囊泡之间的碰撞、囊泡之间自旋标记的交换或囊泡的融合。这些发现证明了在PC双层平面内的快速扩散（横向扩散）。对变宽的进一步研究表明，在0℃时横向扩散的平移步骤的分子频率远大于3×10³秒⁻¹。
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