磷脂在囊泡膜中的侧向扩散。
Lateral diffusion of phospholipids in a vesicle membrane.
作者信息
Kornberg R D, McConnell H M
出版信息
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2564-8. doi: 10.1073/pnas.68.10.2564.
Abstract
Nuclear-resonance spectra of phosphatidylcholine (PC) vesicles are broadened by spin-labeled PC. The broadening of the N-methyl proton line is proportional to spin-labeled PC concentration; the broadening by 1 molecule in 100 of spin-labeled PC is 9.7 Hz at 35 degrees C; the broadening is not due to collisions between vesicles, exchange of spin label between vesicles, or fusion of vesicles. These findings are proof of rapid diffusion in the plane of the PC bilayer (lateral diffusion). A further study of the broadening reveals the molecular frequency of the translation step for lateral diffusion to be much greater than 3 x 10(3) sec(-1) at 0 degrees C.
摘要
磷脂酰胆碱（PC）囊泡的核磁共振谱会因自旋标记的PC而变宽。N - 甲基质子线的变宽与自旋标记的PC浓度成正比；在35℃时，每100个自旋标记的PC分子中有1个导致的变宽为9.7赫兹；这种变宽不是由于囊泡之间的碰撞、囊泡之间自旋标记的交换或囊泡的融合。这些发现证明了在PC双层平面内的快速扩散（横向扩散）。对变宽的进一步研究表明，在0℃时横向扩散的平移步骤的分子频率远大于3×10³秒⁻¹。
相似文献
1
Lateral diffusion of phospholipids in a vesicle membrane.磷脂在囊泡膜中的侧向扩散。
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2564-8. doi: 10.1073/pnas.68.10.2564.
2
Microscopic versus macroscopic diffusion in model membranes by electron spin resonance spectral-spatial imaging.通过电子自旋共振光谱空间成像研究模型膜中的微观与宏观扩散
Biophys J. 1991 Apr;59(4):950-7. doi: 10.1016/S0006-3495(91)82310-4.
3
Study of the transverse diffusion of spin-labeled phospholipids in biological membranes. II. Inner mitochondrial membrane of rat liver: use of phosphatidylcholine exchange protein.自旋标记磷脂在生物膜中横向扩散的研究。II. 大鼠肝脏线粒体内膜：磷脂酰胆碱交换蛋白的应用
Biochim Biophys Acta. 1976 Mar 19;426(3):372-84. doi: 10.1016/0005-2736(76)90383-7.
4
Magnetic resonance spectra of membranes.膜的磁共振光谱
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1451-5. doi: 10.1073/pnas.72.4.1451.
5
Effect of polar carotenoids on the oxygen diffusion-concentration product in lipid bilayers. An EPR spin label study.极性类胡萝卜素对脂质双层中氧扩散 - 浓度积的影响。一项电子顺磁共振自旋标记研究。
Biochim Biophys Acta. 1991 Sep 10;1068(1):68-72. doi: 10.1016/0005-2736(91)90061-c.
6
Bent fatty acid chains in lecithin bilayers.卵磷脂双分子层中弯曲的脂肪酸链。
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1274-8. doi: 10.1073/pnas.68.6.1274.
7
Effects of tumbling and lateral diffusion on phosphatidylcholine model membrane 31P-NMR lineshapes.翻滚和横向扩散对磷脂酰胆碱模型膜31P-NMR线形的影响。
Biochim Biophys Acta. 1980 Dec 2;603(1):63-9. doi: 10.1016/0005-2736(80)90391-0.
8
Measurement of fast lateral diffusion of lipids in vesicles and in biological membranes by 1 H nuclear magnetic resonance.通过氢核磁共振测量囊泡和生物膜中脂质的快速横向扩散。
Biochemistry. 1973 Apr 10;12(8):1650-9. doi: 10.1021/bi00732a029.
9
Electron-electron double resonance and saturation-recovery studies of nitroxide electron and nuclear spin-lattice relaxation times and Heisenberg exchange rates: lateral diffusion in dimyristoyl phosphatidylcholine.氮氧化物电子与核自旋晶格弛豫时间以及海森堡交换率的电子-电子双共振和饱和恢复研究：二肉豆蔻酰磷脂酰胆碱中的横向扩散
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2559-63. doi: 10.1073/pnas.79.8.2559.
10
Effects of paramagnetic shift reagents on the 13C nuclear magnetic resonance spectra of egg phosphatidylcholine enriched with 13C in the N-methyl carbons.顺磁位移试剂对在N-甲基碳中富含¹³C的鸡蛋磷脂酰胆碱的¹³C核磁共振谱的影响。
Biochemistry. 1976 Apr 20;15(8):1635-9. doi: 10.1021/bi00653a007.
引用本文的文献
1
Phosphatidylserine externalization as immune checkpoint in cancer.磷脂酰丝氨酸外化作为癌症中的免疫检查点。
Pflugers Arch. 2024 Dec;476(12):1789-1802. doi: 10.1007/s00424-024-02948-7. Epub 2024 Apr 4.
2
Multilamellar Liposomes as a Model for Biological Membranes: Saturation Recovery EPR Spin-Labeling Studies.多层脂质体作为生物膜模型：饱和恢复电子顺磁共振自旋标记研究
Membranes (Basel). 2022 Jun 26;12(7):657. doi: 10.3390/membranes12070657.
3
Requirement of Xk and Vps13a for the P2X7-mediated phospholipid scrambling and cell lysis in mouse T cells.Xk 和 Vps13a 对 P2X7 介导的小鼠 T 细胞中磷脂重排和细胞裂解的要求。
Proc Natl Acad Sci U S A. 2022 Feb 15;119(7). doi: 10.1073/pnas.2119286119.
4
The tertiary structure of the human Xkr8-Basigin complex that scrambles phospholipids at plasma membranes.人源 Xkr8-Basigin 复合物在质膜上扰乱磷脂的三级结构。
Nat Struct Mol Biol. 2021 Oct;28(10):825-834. doi: 10.1038/s41594-021-00665-8. Epub 2021 Oct 8.
5
Efferocytosis and autoimmune disease.吞噬作用与自身免疫性疾病。
Int Immunol. 2018 Nov 14;30(12):551-558. doi: 10.1093/intimm/dxy055.
6
Phospholipid subcellular localization and dynamics.磷脂亚细胞定位和动态变化。
J Biol Chem. 2018 Apr 27;293(17):6230-6240. doi: 10.1074/jbc.R117.000582. Epub 2018 Mar 27.
7
Phosphoinositides, Major Actors in Membrane Trafficking and Lipid Signaling Pathways.磷酸肌醇，膜运输和脂质信号通路中的主要参与者。
Int J Mol Sci. 2017 Mar 15;18(3):634. doi: 10.3390/ijms18030634.
8
EPR characterization of cellulose triacetate fibers used for enzyme immobilization.用于酶固定化的三醋酸纤维素纤维的 EPR 表征。
Appl Biochem Biotechnol. 1981 Jun;6(2):129-41. doi: 10.1007/BF02779245.
9
Progesterone modulation of transmembrane helix-helix interactions between the alpha-subunit of Na/K-ATPase and phospholipid N-methyltransferase in the oocyte plasma membrane.孕酮对卵母细胞质膜中钠钾ATP酶α亚基与磷脂N-甲基转移酶之间跨膜螺旋-螺旋相互作用的调节。
BMC Struct Biol. 2010 May 25;10:12. doi: 10.1186/1472-6807-10-12.
本文引用的文献
1
A LIGHT-SCATTERING STUDY OF ULTRASONICALLY IRRADIATED LECITHIN SOLS.超声辐照卵磷脂溶胶的光散射研究。
Biochim Biophys Acta. 1965 Apr 5;98:344-50. doi: 10.1016/0005-2760(65)90126-8.
2
Phase equilibria and structure of dry and hydrated egg lecithin.干态和水合态鸡蛋卵磷脂的相平衡与结构
J Lipid Res. 1967 Nov;8(6):551-7.
3
Physical studies of phospholipids. IV. High resolution nuclear magnetic resonance spectra of phospholipids and related substances.磷脂的物理研究。IV. 磷脂及相关物质的高分辨率核磁共振谱
J Biol Chem. 1966 Nov 10;241(21):5044-52.
4
Internal mobility in phospholipids.磷脂的内部流动性。
Nature. 1969 Sep 6;223(5210):1057-9. doi: 10.1038/2231057a0.
5
Studies on phosphatidylcholine vesicles. Formation and physical characteristics.磷脂酰胆碱囊泡的研究。形成与物理特性。
Biochemistry. 1969 Jan;8(1):344-52. doi: 10.1021/bi00829a048.
6
Lipid composition and permeability of liposomes.脂质体的脂质组成与通透性
Biochim Biophys Acta. 1968 Jun 11;150(4):666-75. doi: 10.1016/0005-2736(68)90056-4.
7
Lysolecithin and cell fusion.溶血卵磷脂与细胞融合
Nature. 1970 Aug 22;227(5260):810-4. doi: 10.1038/227810a0.
8
Synthesis of lecithins by acylation of O-(sn-glycero-3-phosphoryl) choline with fatty acid anhydrides.通过用脂肪酸酐酰化O-(sn-甘油-3-磷酸)胆碱合成卵磷脂。
Biochim Biophys Acta. 1969 Dec 17;187(4):520-6. doi: 10.1016/0005-2760(69)90049-6.
9
Inside-outside transitions of phospholipids in vesicle membranes.囊泡膜中磷脂的内外翻转
Biochemistry. 1971 Mar 30;10(7):1111-20. doi: 10.1021/bi00783a003.
Zotero 插件