大肠杆菌K-12泛醌生物合成最终反应受阻的突变体：特性与遗传分析。
Mutants of Escherichia coli K-12 blocked in the final reaction of ubiquinone biosynthesis: characterization and genetic analysis.
作者信息
Stroobant P, Young I G, Gibson F
出版信息
J Bacteriol. 1972 Jan;109(1):134-9. doi: 10.1128/jb.109.1.134-139.1972.
Abstract
The ubiquinone precursor 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1, 4-benzoquinone was isolated from two ubiquinone-deficient mutants of Escherichia coli and identified by nuclear magnetic resonance and mass spectrometry. The results of genetic analysis of the mutants indicate that each mutant carries a mutation in a gene designated ubiG which was located, by cotransduction with the nalA and glpT genes, at minute 42 on the E. coli chromosome.
摘要
从大肠杆菌的两个泛醌缺陷型突变体中分离出泛醌前体2 - 辛戊烯基 - 3 - 甲基 - 5 - 羟基 - 6 - 甲氧基 - 1,4 - 苯醌，并通过核磁共振和质谱进行鉴定。对这些突变体的遗传分析结果表明，每个突变体在一个名为ubiG的基因中携带一个突变，通过与nalA和glpT基因共转导，该基因位于大肠杆菌染色体上的42分钟处。
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