大肠杆菌K-12中泛醌的生物合成：一个无法将分支酸转化为4-羟基苯甲酸的突变体的生化和遗传特征
Biosynthesis of ubiquinone in Escherichia coli K-12: biochemical and genetic characterization of a mutant unable to convert chorismate into 4-hydroxybenzoate.
作者信息
Lawrence J, Cox G B, Gibson F
出版信息
J Bacteriol. 1974 Apr;118(1):41-5. doi: 10.1128/jb.118.1.41-45.1974.
Abstract
A mutant strain of Escherichia coli unable to carry out the first specific reaction of ubiquinone biosynthesis, that is the conversion of chorismate into 4-hydroxybenzoate, has been isolated. The gene concerned maps at about minute 79 on the E. coli chromosome and has been designated ubiC. This gene is probably the structural gene for chorismate lyase since cell extracts from a transductant strain carrying the ubiC437 mutant allele are unable to convert chorismate into 4-hydroxybenzoate and growing cells of the mutant do not form appreciable quantities of ubiquinone unless 4-hydroxybenzoate is added to the growth medium.
摘要
已分离出一种无法进行泛醌生物合成的第一个特定反应（即分支酸转化为4-羟基苯甲酸）的大肠杆菌突变株。相关基因位于大肠杆菌染色体上约79分钟处，已被命名为ubiC。该基因可能是分支酸裂解酶的结构基因，因为携带ubiC437突变等位基因的转导菌株的细胞提取物无法将分支酸转化为4-羟基苯甲酸，并且除非向生长培养基中添加4-羟基苯甲酸，否则该突变株的生长细胞不会形成可观数量的泛醌。
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