Nielsen J O, Elling P
Clin Exp Immunol. 1971 Dec;9(6):699-705.
134 liver biopsies obtained from 124 patients with various diseases of the liver were examined by the direct immunofluorescent technique for the Australia antigen. FITC-labelled IgG fractions prepared from three different sera containing antibody to the Australia antigen were used. Controls included FITC-labelled IgG fractions of sera containing antibodies against human immunoglobulins, complement factors and herpes simplex virus. The three antibodies to the Australia antigen employed showed identical fluorescent patterns and reacted with the same biopsies. In 68 biopsies prepared and examined within 12 hr, speckled or granular fluorescence was noted in the nuclei of the hepatocytes in all cases irrespective of the clinical or histological diagnosis or the serological reaction for the Australia antigen. The antigen in the nuclei of the hepatocytes proved to be very labile. It is concluded that IgG prepared from sera containing antibodies to the Australia antigen may have two antigenic specificities, one precipitating with the Australia antigen in serum and another reacting by immunofluorescence with a labile antigen which appears to be a constituent of normal liver cell nuclei.
采用直接免疫荧光技术，对124例患有各种肝脏疾病的患者所提供的134份肝活检组织进行了澳大利亚抗原检测。使用了由三种不同的含澳大利亚抗原抗体的血清制备的异硫氰酸荧光素（FITC）标记的IgG组分。对照包括含抗人免疫球蛋白、补体因子和单纯疱疹病毒抗体的血清的FITC标记的IgG组分。所使用的三种抗澳大利亚抗原抗体呈现出相同的荧光模式，并与相同的活检组织发生反应。在12小时内制备并检测的68份活检组织中，无论临床诊断、组织学诊断或澳大利亚抗原的血清学反应如何，所有病例的肝细胞细胞核中均观察到斑点状或颗粒状荧光。肝细胞细胞核中的抗原被证明非常不稳定。结论是，由含澳大利亚抗原抗体的血清制备的IgG可能具有两种抗原特异性，一种与血清中的澳大利亚抗原沉淀，另一种通过免疫荧光与一种不稳定抗原反应，该不稳定抗原似乎是正常肝细胞核的一种成分。
