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通过免疫荧光法对澳大利亚抗原在肝脏中的定位。

The localization of the Australia antigen in the liver by immunofluorescence.

作者信息

Akeyama T, Kamada T, Koizumi T, Abe H

出版信息

J Clin Pathol. 1972 Dec;25(12):1071-4. doi: 10.1136/jcp.25.12.1071.

DOI:10.1136/jcp.25.12.1071
PMID:4570618
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC477623/
Abstract

The localization of Australia antigen in the liver by the fluorescent antibody technique (direct method) has been studied. Specific fluorescence was observed in the cytoplasm of liver cells. Two distribution patterns of fluorescent-positive cells, namely, a diffuse fluorescence in the liver lobules and a spotty distribution of fluorescent-positive cells, were observed.

摘要

采用荧光抗体技术(直接法)对澳大利亚抗原在肝脏中的定位进行了研究。在肝细胞的细胞质中观察到特异性荧光。观察到荧光阳性细胞的两种分布模式,即肝小叶中的弥漫性荧光和荧光阳性细胞的斑点状分布。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7743/477623/c58043c02b34/jclinpath00110-0061-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7743/477623/65a52658b3bb/jclinpath00110-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7743/477623/39ad568e14c2/jclinpath00110-0060-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7743/477623/f890a582a338/jclinpath00110-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7743/477623/c58043c02b34/jclinpath00110-0061-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7743/477623/65a52658b3bb/jclinpath00110-0060-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7743/477623/39ad568e14c2/jclinpath00110-0060-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7743/477623/f890a582a338/jclinpath00110-0061-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7743/477623/c58043c02b34/jclinpath00110-0061-b.jpg

相似文献

1
The localization of the Australia antigen in the liver by immunofluorescence.通过免疫荧光法对澳大利亚抗原在肝脏中的定位。
J Clin Pathol. 1972 Dec;25(12):1071-4. doi: 10.1136/jcp.25.12.1071.
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Intrahepatic localisation of Australia antigen by fluorescent antibody technique.用荧光抗体技术对澳大利亚抗原进行肝内定位
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[Localization of HBsAg, immunoglobulins, fibrin and C3 in the liver by direct immunoflourescence].[通过直接免疫荧光法对肝脏中乙肝表面抗原、免疫球蛋白、纤维蛋白和C3进行定位]
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Evaluation of a modified fluorescent technique for the detection of Australia antigen in liver tissue.一种改良荧光技术用于检测肝组织中澳大利亚抗原的评估。
J Immunol Methods. 1974 Jan;3(1):47-52. doi: 10.1016/0022-1759(74)90032-5.
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Detection of Australia antigen in liver biopsies by immunofluorescence.通过免疫荧光法检测肝活检组织中的澳大利亚抗原。
Can Med Assoc J. 1973 Apr 21;108(8):981-4.

引用本文的文献

1
The diagnostic significance of intrahepatocellular hepatitis-B-surface-antigen (HBsAg), hepatitis-B-core-antigen (HBcAg) and IgG for the classification of inflammatory liver diseases. (Studies on HBsAg-positive and -negative patients).肝细胞内乙型肝炎表面抗原(HBsAg)、乙型肝炎核心抗原(HBcAg)及IgG对炎症性肝病分类的诊断意义。(对HBsAg阳性和阴性患者的研究)
Klin Wochenschr. 1975 Nov 15;53(22):1069-74. doi: 10.1007/BF01614383.
2
[Investigations concerning the fixation of hepatitis B-antigen (HBAg) on peripheral lymphocytes and isolated hepatocytes in patients with inflammatory liver diseases. (author's transl)].关于炎症性肝病患者外周淋巴细胞和分离肝细胞上乙肝抗原(HBAg)固定情况的研究。(作者译)
Klin Wochenschr. 1975 Mar 1;53(5):231-5. doi: 10.1007/BF01468812.
3

本文引用的文献

1
FACTORS AFFECTING THE RATE OF REACTION OF FLUORESCEIN ISOTHIOCYANATE WITH SERUM PROTEINS.影响异硫氰酸荧光素与血清蛋白反应速率的因素
J Immunol. 1964 Aug;93:232-42.
2
The chromatographic purification of fluorescein-antibody.荧光素抗体的色谱纯化
J Histochem Cytochem. 1961 Sep;9:484-6. doi: 10.1177/9.5.484.
3
Studies on fluorescent antibody staining. I. Non-specific fluorescence with fluorescein-coupled sheep anti-rabbit globulins.荧光抗体染色研究。I. 荧光素偶联羊抗兔球蛋白的非特异性荧光。
[The characterization of clinically healthy hepatitis-b-surface-antigen (HBsAg)-carriers. Clinical, biochemical, histiological and immunological investigations in 129 case of a prospective study (author's transl)].[临床健康的乙型肝炎表面抗原(HBsAg)携带者的特征。一项前瞻性研究中129例病例的临床、生化、组织学和免疫学调查(作者译)]
Klin Wochenschr. 1976 Jun 15;54(12):567-78. doi: 10.1007/BF01619572.
4
Immunohistochemical patterns of hepatitis B surface antigen (HBsAg) in patients with hepatitis, renal homografts recipients and normal carriers.肝炎患者、肾移植受者及正常携带者中乙型肝炎表面抗原(HBsAg)的免疫组化模式。
Virchows Arch A Pathol Anat Histol. 1977 Dec 8;376(4):329-41. doi: 10.1007/BF00432302.
5
Methods for detection of hepatitis B surface antigen in paraffin sections of liver: a guideline for their use.肝脏石蜡切片中乙型肝炎表面抗原的检测方法:使用指南
J Clin Pathol. 1977 May;30(5):460-3. doi: 10.1136/jcp.30.5.460.
6
Demonstration of hepatitis B surface antigen by orcein staining in paraffin sections of cirrhotic liver.在肝硬化肝脏石蜡切片中通过地衣红染色显示乙型肝炎表面抗原。
Virchows Arch A Pathol Anat Histol. 1976 Feb 16;369(3):239-48. doi: 10.1007/BF00427712.
J Exp Med. 1961 Jul 1;114(1):89-110. doi: 10.1084/jem.114.1.89.
4
Superiority of fluorescein isothiocyanate (Riggs) for fluorescent-antibody technic with a modification of its application.异硫氰酸荧光素(里格斯)在荧光抗体技术中的优势及其应用的改进
Proc Soc Exp Biol Med. 1958 Aug-Sep;98(4):898-900. doi: 10.3181/00379727-98-24222.
5
Australia antigen and hepatitis. An electron microscopic study.澳大利亚抗原与肝炎。一项电子显微镜研究。
Arch Pathol. 1971 Jul;92(1):66-72.
6
[Ultrastructural study of unusual cytoplasmic inclusions in viral hepatitis. Relation with Australia antigen?].[病毒性肝炎中异常细胞质内含物的超微结构研究。与澳大利亚抗原的关系?]
Presse Med (1893). 1970 Nov 7;78(47):2061-4.
7
The localization of Australia antigen by immunofluorescence.通过免疫荧光法对澳大利亚抗原进行定位。
J Exp Med. 1970 Feb;131(2):307-19. doi: 10.1084/jem.131.2.307.
8
Investigations of liver biopsies for Australia antigen by immunofluorescent technique.采用免疫荧光技术对澳大利亚抗原进行肝活检研究。
Clin Exp Immunol. 1971 Dec;9(6):699-705.
9
Cellular localisation of Australia antigen in the liver of patients with lymphoproliferative disorders.澳大利亚抗原在淋巴增生性疾病患者肝脏中的细胞定位。
Lancet. 1970 Mar 7;1(7645):494-8. doi: 10.1016/s0140-6736(70)91580-1.
10
Intrahepatic expression of serum hepatitis virus-associated antigens.血清型肝炎病毒相关抗原的肝内表达
J Exp Med. 1971 Oct 1;134(4):871-85. doi: 10.1084/jem.134.4.871.