含有化学修饰3'末端5S RNA的核糖体活性。
Activity of ribosomes containing 5S RNA with a chemically modified 3'-terminus.
作者信息
Fahnestock S R, Nomura M
出版信息
Proc Natl Acad Sci U S A. 1972 Feb;69(2):363-5. doi: 10.1073/pnas.69.2.363.
Abstract
Ribosomes containing 5S RNA in which the 3'-terminus has been modified chemically, either by periodate oxidation or by conversion of the terminal ribose to a morpholine derivative, are fully as active in peptidebond formation and polypeptide synthesis as those ribosomes with unmodified 5S RNA. These results exclude models for protein synthesis in which peptidyl-5S RNA is formed as an intermediate.
摘要
含有经化学修饰的5S RNA的核糖体，其3'末端已通过高碘酸盐氧化或通过将末端核糖转化为吗啉衍生物进行了化学修饰，在肽键形成和多肽合成方面与具有未修饰5S RNA的核糖体一样活跃。这些结果排除了以肽基-5S RNA作为中间体形成的蛋白质合成模型。
相似文献
1
Activity of ribosomes containing 5S RNA with a chemically modified 3'-terminus.含有化学修饰3'末端5S RNA的核糖体活性。
Proc Natl Acad Sci U S A. 1972 Feb;69(2):363-5. doi: 10.1073/pnas.69.2.363.
2
Reconstitution of 50S ribosomal subunits and the role of 5S RNA.
Basic Life Sci. 1973;1:241-50. doi: 10.1007/978-1-4684-0877-5_20.
3
Role of 5S RNA in the functions of 50S ribosomal subunits.5S RNA在50S核糖体亚基功能中的作用。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):2932-6. doi: 10.1073/pnas.68.12.2932.
4
30S ribosomal proteins cross-linked to 16S RNA by periodate oxidation followed by borohydride reduction.通过高碘酸盐氧化，然后硼氢化钠还原，使30S核糖体蛋白与16S RNA交联。
Mol Biol Rep. 1978 Oct 16;4(3):153-6. doi: 10.1007/BF00777516.
5
Crosslinking of proteins to ribosomal RNA in HeLa cell polysomes by sodium periodate.高碘酸钠介导的HeLa细胞多核糖体中蛋白质与核糖体RNA的交联作用
Biochem Biophys Res Commun. 1978 Apr 28;81(4):1145-52. doi: 10.1016/0006-291x(78)91256-1.
6
How ribosomes select initiator regions in mRNA: base pair formation between the 3' terminus of 16S rRNA and the mRNA during initiation of protein synthesis in Escherichia coli.核糖体如何在mRNA中选择起始区域：大肠杆菌蛋白质合成起始过程中16S rRNA的3'末端与mRNA之间的碱基对形成
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4734-8. doi: 10.1073/pnas.72.12.4734.
7
Chemical reactivity of E. coli 5S RNA in situ in the 50S ribosomal subunit.大肠杆菌5S RNA在50S核糖体亚基中的原位化学反应活性。
Nucleic Acids Res. 1983 Feb 11;11(3):605-17. doi: 10.1093/nar/11.3.605.
8
In vitro incorporation of eubacterial, archaebacterial and eukaryotic 5S rRNAs into large ribosomal subunits of Bacillus stearothermophilus.嗜热脂肪芽孢杆菌大核糖体亚基中外源真细菌、古细菌和真核生物5S rRNA的体外掺入
Nucleic Acids Res. 1988 Apr 25;16(8):3511-24. doi: 10.1093/nar/16.8.3511.
9
Conformation of 4.5S RNA in the signal recognition particle and on the 30S ribosomal subunit.信号识别颗粒及30S核糖体亚基中4.5S RNA的构象
RNA. 2005 Sep;11(9):1374-84. doi: 10.1261/rna.7219805. Epub 2005 Jul 25.
10
Fluorescence studies of the accessibility of the 3' ends of the ribosomal RNAs in Escherichia coli ribosomes and subunits.大肠杆菌核糖体及亚基中核糖体RNA 3'末端可及性的荧光研究。
Nucleic Acids Res. 1979 Jan;6(1):181-93. doi: 10.1093/nar/6.1.181.
引用本文的文献
1
Functional group recognition at the aminoacylation and editing sites of E. coli valyl-tRNA synthetase.大肠杆菌缬氨酰 - tRNA合成酶氨酰化和校对位点的功能基团识别
RNA. 2004 Mar;10(3):493-503. doi: 10.1261/rna.5166704.
2
Differential utilization of poly(rC) binding protein 2 in translation directed by picornavirus IRES elements.微小核糖核酸病毒内部核糖体进入位点元件介导的翻译过程中多聚（rC）结合蛋白2的差异利用
RNA. 1999 Dec;5(12):1570-85. doi: 10.1017/s1355838299991483.
3
Poly(rC) binding protein 2 binds to stem-loop IV of the poliovirus RNA 5' noncoding region: identification by automated liquid chromatography-tandem mass spectrometry.聚（rC）结合蛋白2与脊髓灰质炎病毒RNA 5'非编码区的茎环IV结合：通过自动液相色谱-串联质谱法鉴定。
Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):11115-20. doi: 10.1073/pnas.93.20.11115.
4
Expression of ribosomal protein genes as analyzed by bacteriophage Mu-induced mutations.通过噬菌体Mu诱导的突变分析核糖体蛋白基因的表达。
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1526-30. doi: 10.1073/pnas.69.6.1526.
5
Affinity chromatography with an immobilized RNA enzyme.使用固定化RNA酶的亲和色谱法。
Proc Natl Acad Sci U S A. 1986 Aug;83(16):5904-8. doi: 10.1073/pnas.83.16.5904.
6
An RNA molecule copurifies with RNase P activity from Xenopus laevis oocytes.一种RNA分子与来自非洲爪蟾卵母细胞的RNase P活性共同纯化。
Nucleic Acids Res. 1991 May 11;19(9):2315-20. doi: 10.1093/nar/19.9.2315.
7
Surface topography of the Bacillus stearothermophilus ribosome.嗜热脂肪芽孢杆菌核糖体的表面形貌
Mol Gen Genet. 1976 Mar 30;144(3):273-80. doi: 10.1007/BF00341725.
本文引用的文献
1
2
The reaction of methylamine with periodate-oxidized adenosine 5'-phosphate.甲胺与高碘酸盐氧化的腺苷5'-磷酸的反应。
Biochemistry. 1963 Mar-Apr;2:344-50. doi: 10.1021/bi00902a029.
3
A model for protein synthesis involving the intermediate formation of peptidyl-5S RNA.一种涉及肽基-5S RNA中间形成的蛋白质合成模型。
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2357-60. doi: 10.1073/pnas.68.10.2357.
4
Role of 5S RNA in the functions of 50S ribosomal subunits.5S RNA在50S核糖体亚基功能中的作用。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):2932-6. doi: 10.1073/pnas.68.12.2932.
5
Inactivation and reactivation of ribosomal subunits: the peptidyl transferase activity of the 50 s subunit of Escherihia coli.核糖体亚基的失活与再激活：大肠杆菌50 s亚基的肽基转移酶活性
J Mol Biol. 1970 Dec 14;54(2):355-78. doi: 10.1016/0022-2836(70)90435-3.
Zotero 插件