Celma M L, Ehrenfeld E
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2440-4. doi: 10.1073/pnas.71.6.2440.
Cell-free protein-synthesizing systems that initiate on endogenous messenger RNA have been developed from uninfected and poliovirus-infected HeLa cells. Poliovirus double-stranded RNA is an effective inhibitor of protein synthesis in these extracts, and both cell-directed and virus-specific protein synthesis are equally sensitive to the inhibitory action of double-stranded RNA. The concentrations of double-stranded RNA required for inhibition are not achieved in the infected cell at early times after infection when host-cell shut-off occurs, but rather are achieved only late in infection when virus-specific protein synthesis begins to decline. This indicates that double-stranded RNA does not act as a direct agent to inhibit host cell protein synthesis following infection by poliovirus. The possible significance of inhibition by double-stranded RNA of poliovirus-specific protein synthesis is discussed.
已从未感染和脊髓灰质炎病毒感染的海拉细胞中开发出以内源性信使核糖核酸起始的无细胞蛋白质合成系统。脊髓灰质炎病毒双链核糖核酸是这些提取物中蛋白质合成的有效抑制剂，细胞导向的和病毒特异性的蛋白质合成对双链核糖核酸的抑制作用同样敏感。在感染后宿主细胞关闭的早期，感染细胞中并未达到抑制所需的双链核糖核酸浓度，而是仅在感染后期病毒特异性蛋白质合成开始下降时才达到。这表明双链核糖核酸在脊髓灰质炎病毒感染后并非作为直接因子抑制宿主细胞蛋白质合成。本文讨论了双链核糖核酸对脊髓灰质炎病毒特异性蛋白质合成抑制的可能意义。
