Tesh R B, Chaniotis B N, Johnson K M
Science. 1972 Mar 31;175(4029):1477-9. doi: 10.1126/science.175.4029.1477.
Transovarial transmission of vesicular stomatitis virus (Indiana serotype) by experimentally infected Lutzomyia trapidoi and Lutzomyia ylephiletrix to their progeny was demonstrated. Virus was recovered from all developmental stages; mean virus titers from egg to first generation adult showed a four-log increase, indicating that virus multiplication occurred during development of the sandflies. Virus titers in first generation adult females were comparable to those found in their parents. These infected female sandflies transmitted vesicular stomatitus virus Indiana by bite to susceptible animals and transmitted the virus transovarially to their offspring (second generation). Results demonstrate a possible mechanism for transmission and maintenance of this virus in nature without a vertebrate (heat) host reservoir.
实验证明，受水疱性口炎病毒（印第安纳血清型）感染的特拉氏白蛉和耶氏白蛉可将该病毒经卵传递给后代。在所有发育阶段均检测到病毒；从卵到第一代成虫，病毒平均滴度增加了4个对数，表明病毒在白蛉发育过程中发生了增殖。第一代成年雌性白蛉体内的病毒滴度与它们父母体内的相当。这些受感染的雌性白蛉通过叮咬将印第安纳水疱性口炎病毒传播给易感动物，并将病毒经卵传递给它们的后代（第二代）。结果表明，在没有脊椎动物（温热）宿主储存库的情况下，该病毒在自然界中可能存在一种传播和维持机制。
