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携带小鼠肉瘤病毒基因组的仓鼠肿瘤细胞（HT-1细胞）的细胞化学、细胞遗传学及超微结构

Cytochemistry, cytogenetics and ultrastructure of hamster tumour cells carrying mouse sarcoma viral genome (HT-1 cells).

作者信息

Karpas A, Cawley J, Tuckerman E, Flemans R, Hayhoe F G

出版信息

Br J Cancer. 1971 Dec;25(4):779-88. doi: 10.1038/bjc.1971.92.

DOI:10.1038/bjc.1971.92
PMID:4335635
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2008861/
Abstract

The pleomorphic cytology of the HT-1 cell line is described. Cytochemical studies indicated the presence of glycogen and lysosomes in these cells. Cytogenetic studies demonstrated the presence of a large telocentric chromosome (M1) and two minute chromosomes (M2) not found in normal hamster cells. A cloned line was isolated which did not contain these marker chromosomes. All cells were hyperdiploid with chromosome number around triploidy, although none was a true triploid. Ultrastructural studies revealed the presence of "nuclear bodies", "dense bodies" and "interchromatinic granules" which have been previously described in viral induced malignancies. A few of the cells contained in their cytoplasm structures of approximately 700 [unk] in diameter which bore some resemblance to immature virus particles. Both light and electron microscopy demonstrated some nuclei lacking chromatin aggregates. This is interpreted to mean that the total genetic material of these nuclei was dispersed as free DNA not linked with histone to form chromatin aggregates.

摘要

描述了HT - 1细胞系的多形性细胞学特征。细胞化学研究表明这些细胞中存在糖原和溶酶体。细胞遗传学研究证明存在一条正常仓鼠细胞中未发现的大型端着丝粒染色体（M1）和两条微小染色体（M2）。分离出了一个不含有这些标记染色体的克隆系。所有细胞均为超二倍体，染色体数约为三倍体，但没有一个是真正的三倍体。超微结构研究揭示了“核体”、“致密体”和“染色质间颗粒”的存在，这些在病毒诱导的恶性肿瘤中已有描述。一些细胞的细胞质中含有直径约700 [未知单位] 的结构，与未成熟病毒颗粒有一些相似之处。光学显微镜和电子显微镜均显示一些细胞核缺乏染色质聚集体。这被解释为意味着这些细胞核的全部遗传物质以游离DNA的形式分散，未与组蛋白连接形成染色质聚集体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/37393063f551/brjcancer00361-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/1385557d8878/brjcancer00361-0203-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/724c0c3007c1/brjcancer00361-0206-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/fe36c6bfe52e/brjcancer00361-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/f05fac098353/brjcancer00361-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/d128bbb82357/brjcancer00361-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/2d5284a92274/brjcancer00361-0208-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/d1eacfbb3563/brjcancer00361-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/d1c81cd3b508/brjcancer00361-0213-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/1146f2915055/brjcancer00361-0199-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/46ad7ff50f24/brjcancer00361-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/26eccd07bfdb/brjcancer00361-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/0b67ad241b55/brjcancer00361-0204-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/895f3a512458/brjcancer00361-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/8c84e75502e7/brjcancer00361-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/37393063f551/brjcancer00361-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/1385557d8878/brjcancer00361-0203-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/724c0c3007c1/brjcancer00361-0206-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/fe36c6bfe52e/brjcancer00361-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/f05fac098353/brjcancer00361-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/d128bbb82357/brjcancer00361-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/2d5284a92274/brjcancer00361-0208-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/d1eacfbb3563/brjcancer00361-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/d1c81cd3b508/brjcancer00361-0213-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/1146f2915055/brjcancer00361-0199-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/46ad7ff50f24/brjcancer00361-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/26eccd07bfdb/brjcancer00361-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/0b67ad241b55/brjcancer00361-0204-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/895f3a512458/brjcancer00361-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/8c84e75502e7/brjcancer00361-0205-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50ce/2008861/37393063f551/brjcancer00361-0209-a.jpg

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