Norval M, Marmion B P
Ann Rheum Dis. 1976 Apr;35(2):106-13. doi: 10.1136/ard.35.2.106.
Synovial fibroblast cell strains derived from the synovial membranes of 7 patients with rheumatoid arthritis were examined for the presence of viruses, in particular leucoviruses. Seven similar synovial strains derived from patients with other arthritic conditions were used as a control group. Evidence of the presence of a virus or a viral genome was looked for by several methods of induction followed by 3H-uridine labelling of the cultures. In addition, the culture supernatant, after induction and after the synovial strains had been co-cultivated with a variety of cell lines from several species, was assayed for the presence of viral RNA-dependent DNA polymerase activity. The DNA-polymerase activity of the synovial cells themselves was also determined. No evidence was found by any of these techniques to indicate the presence of virus or viral information within the synovial fibroblasts.
对从7例类风湿性关节炎患者滑膜中分离出的滑膜成纤维细胞系进行了病毒检测，尤其是白血病病毒。从其他关节炎患者中分离出的7个类似滑膜细胞系用作对照组。通过几种诱导方法，随后用3H-尿苷对培养物进行标记，寻找病毒或病毒基因组存在的证据。此外，在诱导后以及滑膜细胞系与来自几个物种的多种细胞系共培养后，检测培养上清液中是否存在病毒RNA依赖性DNA聚合酶活性。还测定了滑膜细胞本身的DNA聚合酶活性。通过这些技术均未发现任何证据表明滑膜成纤维细胞内存在病毒或病毒信息。
