Belfrage P, Johansson B W, Torp A, Akesson B
Acta Med Scand. 1979;205(4):283-6. doi: 10.1111/j.0954-6820.1979.tb06048.x.
Triglycerides and phospholipids extracted from endomyocardial biopsy specimens were quantitated with sensitive photometric methods and the fatty acid composition of phospholipids was determined after separation of the methyl esters by gas chromatography. The degree of triglyceride accumulation was expressed as the molar ratio triglyceride/phospholipid since in most cases the phospholipid concentration is relatively constant. The representativity of the analysis of endomyocardial biopsy was investigated by comparing lipid analyses of large and small myocardial specimens obtained at necropsy. The methods described may be valuable tools in studies on metabolic disturbances in different cardiomyophaties.
采用灵敏的光度法对取自心内膜心肌活检标本中的甘油三酯和磷脂进行定量分析，并在通过气相色谱法分离甲酯后测定磷脂的脂肪酸组成。由于在大多数情况下磷脂浓度相对恒定，所以甘油三酯的积累程度以甘油三酯/磷脂的摩尔比来表示。通过比较尸检时获得的大小不同心肌标本的脂质分析，研究了心内膜心肌活检分析的代表性。所述方法可能是研究不同心肌病代谢紊乱的有价值工具。
