Rocquelin G, Guenot L, Justrabo E, Grynberg A, David M
J Mol Cell Cardiol. 1985 Aug;17(8):769-73. doi: 10.1016/s0022-2828(85)80038-9.
Fatty acid composition of human heart phospholipids was determined in 53 specimens of left ventricular myocardium collected during mitral valve replacement. Ages of the subjects (29 males and 24 females) ranged from 14 to 75 years (mean age = 54). Samples were immediately placed in chloroform-methanol 2/1, v/v to which antioxidant was added. Extracted phospholipids were converted to methyl esters which were analyzed by gas liquid chromatography on glass capillary columns. Morphological examination was also performed on 35 out of 53 samples. Age of the patients as well as the morphological state of the organ had no significant effect on major fatty acids in heart phospholipids. No difference by sex was detected. Trans-octadecenoic isomers were detected in all samples but they remained at a low level (0.4% to 1.2% of the total fatty acids).
在二尖瓣置换手术期间收集的53份左心室心肌样本中，测定了人心磷脂的脂肪酸组成。受试者（29名男性和24名女性）年龄在14至75岁之间（平均年龄 = 54岁）。样本立即置于添加了抗氧化剂的氯仿 - 甲醇（2/1，v/v）中。提取的磷脂转化为甲酯，通过玻璃毛细管柱上的气相色谱法进行分析。53个样本中的35个还进行了形态学检查。患者年龄以及器官的形态状态对心磷脂中的主要脂肪酸没有显著影响。未检测到性别差异。在所有样本中均检测到反式十八碳烯酸异构体，但它们的含量保持在较低水平（占总脂肪酸的0.4%至1.2%）。
