Carbonell L M, Berliner M D, Gil F
J Bacteriol. 1972 May;110(2):731-8. doi: 10.1128/jb.110.2.731-738.1972.
Protoplast formation by Histoplasma capsulatum yeasts using high concentrations of MgSO(4) occurs either by lysis of the bud or lysis of the entire cell wall. Both mechanisms may also occur simultaneously. Neither the protoplast emerging through a hole in the cell wall nor the freshly released protoplast has a recognizable cell wall or the remnant of such. The protoplast contains all the organelles of the normal cell except for mesosomes. During protoplast formation the nucleus increases in size and produces several nuclear masses by the invaginations of the internal layer of the nuclear membrane. All these nuclear masses are surrounded by the external layer of the nuclear membrane. Several nuclei with a normal nuclear membrane are formed later.
荚膜组织胞浆菌酵母利用高浓度硫酸镁形成原生质体的过程，要么通过芽体裂解，要么通过整个细胞壁裂解。这两种机制也可能同时发生。无论是通过细胞壁上的孔洞出现的原生质体，还是刚释放的原生质体，都没有可识别的细胞壁或其残余部分。原生质体包含正常细胞的所有细胞器，除了中介体。在原生质体形成过程中，细胞核体积增大，并通过核膜内层的内陷产生几个核团。所有这些核团都被核膜外层包围。后来形成了几个具有正常核膜的细胞核。
