RNA肿瘤病毒的基因组含有聚腺苷酸序列。
The genome of RNA tumor viruses contains polyadenylic acid sequences.
作者信息
Green M, Cartas M
出版信息
Proc Natl Acad Sci U S A. 1972 Apr;69(4):791-4. doi: 10.1073/pnas.69.4.791.
The 70S genome of two RNA tumor viruses, murine sarcoma virus and avian myeloblastosis virus, binds to Millipore filters in buffer with high salt concentration and to glass fiber filters containing poly(U). These observations suggest that 70S RNA contains adenylic acid-rich sequences. When digested by pancreatic RNase, 70S RNA of murine sarcoma virus yielded poly(A) sequences that contain 91% adenylic acid. These poly(A) sequences sedimented as a relatively homogenous peak in sucrose gradients with a sedimentation coefficient of 4-5 S, but had a mobility during polyacrylamide gel electrophoresis that corresponds to molecules that sediment at 6-7 S. If we estimate a molecular weight for each sequence of 30,000-60,000 (100-200 nucleotides) and a molecular weight for viral 70S RNA of 3-12 million, each viral genome could contain 1-8 poly(A) sequences. Possible functions of poly(A) in the infecting viral RNA may include a role in the initiation of viral DNA or RNA synthesis, in protein maturation, or in the assembly of the viral genome.
两种RNA肿瘤病毒——鼠肉瘤病毒和禽成髓细胞瘤病毒的70S基因组，在高盐浓度缓冲液中能与密理博过滤器结合，也能与含有聚尿苷酸的玻璃纤维过滤器结合。这些观察结果表明，70S RNA含有富含腺苷酸的序列。当用胰核糖核酸酶消化时，鼠肉瘤病毒的70S RNA产生了含有91%腺苷酸的聚腺苷酸序列。这些聚腺苷酸序列在蔗糖梯度中以相对均匀的峰沉降，沉降系数为4 - 5 S，但在聚丙烯酰胺凝胶电泳中的迁移率与沉降系数为6 - 7 S的分子相对应。如果我们估计每个序列的分子量为30,000 - 60,000（100 - 200个核苷酸），病毒70S RNA的分子量为300万 - 1200万，那么每个病毒基因组可能包含1 - 8个聚腺苷酸序列。聚腺苷酸在感染性病毒RNA中的可能功能可能包括在病毒DNA或RNA合成起始、蛋白质成熟或病毒基因组组装中发挥作用。
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