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1
Characterization of a protein species isolated from HeLa cell cytoplasm by affinity chromatography on polyadenylate-sepharose.通过聚腺苷酸-琼脂糖亲和层析从人宫颈癌（HeLa）细胞胞质中分离出的一种蛋白质的特性分析。
Proc Natl Acad Sci U S A. 1974 May;71(5):1882-6. doi: 10.1073/pnas.71.5.1882.
2
Purification and partial characterization of a 19kD/pI 4.5 nucleolar phosphoprotein.一种19kD/pI 4.5核仁磷蛋白的纯化及部分特性分析
Biochem Biophys Res Commun. 1984 Jul 18;122(1):47-55. doi: 10.1016/0006-291x(84)90437-6.
3
Physicochemical properties of the cytoplasmic glucocorticoid receptor complex in HeLa S3 cells.HeLa S3细胞中细胞质糖皮质激素受体复合物的物理化学性质
J Steroid Biochem. 1982 Mar;16(3):419-28. doi: 10.1016/0022-4731(82)90055-3.
4
Isolation of messenger RNA from polysomes by chromatography on oligo(dT)-cellulose.通过在寡聚（dT）-纤维素上进行层析从多核糖体中分离信使核糖核酸。
Anal Biochem. 1975 May 26;66(1):18-28. doi: 10.1016/0003-2697(75)90720-4.
5
Ribonucleoprotein organization of polyadenylate sequences in HeLa cell heterogeneous nuclear RNA.HeLa细胞异质核RNA中聚腺苷酸序列的核糖核蛋白组织
J Mol Biol. 1975 Jun 25;95(2):227-38. doi: 10.1016/0022-2836(75)90392-7.
6
[Isolation and characterization of glycerol dehydrogenase from bacillus megaterium].[巨大芽孢杆菌甘油脱氢酶的分离与鉴定]
Hoppe Seylers Z Physiol Chem. 1983 Jul;364(7):911-21.
7
Biochemical characterization of mouse brain necdin.小鼠脑Necdin的生化特性
Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3):895-901. doi: 10.1042/bj3140895.
8
Isolation of human urinary kallikrein by affinity chromatography. Determination of human urinary kallikrein, I.通过亲和色谱法分离人尿激肽释放酶。人尿激肽释放酶的测定，I。
J Clin Chem Clin Biochem. 1977 Sep;15(9):479-83. doi: 10.1515/cclm.1977.15.1-12.479.
9
Properties of a 5'-nucleotidase purified from mouse liver plasma membranes.从小鼠肝细胞膜中纯化的5'-核苷酸酶的性质。
Biochem J. 1973 May;133(1):189-99. doi: 10.1042/bj1330189.
10
Studies on human antihemophilic factor. Evidence for a covalently linked subunit structure.人类抗血友病因子的研究。共价连接亚基结构的证据。
J Clin Invest. 1976 Apr;57(4):925-37. doi: 10.1172/JCI108369.

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Finding one's way in proteomics: a protein species nomenclature.蛋白质组学中的探索之路：一种蛋白质种类命名法。
Chem Cent J. 2009 Sep 9;3:11. doi: 10.1186/1752-153X-3-11.
2
RNA-binding properties of a translational activator, the adenovirus L4 100-kilodalton protein.一种翻译激活因子——腺病毒L4 100千道尔顿蛋白的RNA结合特性。
J Virol. 1993 Jun;67(6):3586-95. doi: 10.1128/JVI.67.6.3586-3595.1993.
3
Developmental changes in the protein and ribonucleic acid components of rat brain messenger ribonucleic acid-protein particles isolated from free polyribosomes by oligo(dT)-cellulose chromatography.通过寡聚（dT）-纤维素色谱法从游离多核糖体中分离出的大鼠脑信使核糖核酸-蛋白质颗粒的蛋白质和核糖核酸成分的发育变化。
Biochem J. 1980 Jul 15;190(1):215-23. doi: 10.1042/bj1900215.
4
Extreme instability of myc mRNA in normal and transformed human cells.在正常和转化的人类细胞中，myc信使核糖核酸（mRNA）具有极高的不稳定性。
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7046-50. doi: 10.1073/pnas.81.22.7046.
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Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities.通过RNA结合特异性对不均一核核糖核蛋白颗粒的蛋白质进行分类和纯化。
Mol Cell Biol. 1988 May;8(5):2237-41. doi: 10.1128/mcb.8.5.2237-2241.1988.
6
Interaction of poly(A) and mRNA with eukaryotic initiator met-tRNA-f binding factor: identification of this activity on reticulocyte ribonucleic acid protein particles.聚腺苷酸（poly(A)）与真核起始甲硫氨酰 - tRNA - f结合因子与mRNA的相互作用：在网织红细胞核糖核酸蛋白颗粒上对该活性的鉴定。
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1021-5. doi: 10.1073/pnas.72.3.1021.
7
Nuclear proteins. II. Similarity of nonhistone proteins in nuclear sap and chromatin, and essential absence of contractile proteins from mouse liver nuclei.核蛋白。II. 核液和染色质中非组蛋白的相似性，以及小鼠肝细胞核中基本不存在收缩蛋白。
J Cell Biol. 1976 Aug;70(2 pt 1):440-52. doi: 10.1083/jcb.70.2.440.
8
Messenger ribonucleoprotein complexes of cryptobiotic embryos of Artemia salina.卤虫隐生胚胎的信使核糖核蛋白复合体
Nucleic Acids Res. 1977 Mar;4(3):625-39. doi: 10.1093/nar/4.3.625.
9
Preparation of poly (A)-binding proteins from endoplasmic reticulum-containing subfractions of rat liver cells and their use in mRNA purification.从大鼠肝细胞含内质网亚组分中制备聚（A）结合蛋白及其在mRNA纯化中的应用。
Mol Cell Biochem. 1977 Mar 21;15(1):67-71. doi: 10.1007/BF01731290.
10
Characterization of protein components of poly(A)-containing messenger ribonucleoproteins from cryptobiotic gastrulae of Artemia salina.卤虫隐生胚囊中含聚腺苷酸信使核糖核蛋白的蛋白质成分的表征
Mol Biol Rep. 1979 May 31;5(1-2):65-9. doi: 10.1007/BF00777490.

本文引用的文献

1
AN ENDONUCLEASE FROM NEUROSPORA CRASSA SPECIFIC FOR POLYNUCLEOTIDES LACKING AN ORDERED STRUCTURE. I. PURIFICATION AND PROPERTIES OF THE ENZYME.一种来自粗糙脉孢菌的核酸内切酶，对缺乏有序结构的多核苷酸具有特异性。I. 酶的纯化及性质
J Biol Chem. 1965 Mar;240:1287-93.
2
Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. IV. Further studies on the resolving power in connection with separation of myoglobins.天然pH梯度中两性电解质的等电分级分离、分析及特性鉴定。IV. 与肌红蛋白分离相关的分辨率的进一步研究。
Acta Chem Scand. 1966;20(3):820-34. doi: 10.3891/acta.chem.scand.20-0820.
3
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
4
[40 S ribonucleoprotein particles in rat liver nuclei].大鼠肝细胞核中的40 S核糖核蛋白颗粒
J Mol Biol. 1968 Apr 28;33(2):465-81. doi: 10.1016/0022-2836(68)90203-9.
5
Particles of nuclear origin carrying rapidly labelled RNA.携带快速标记RNA的核源颗粒。
Eur J Biochem. 1968 Sep 24;5(4):500-6. doi: 10.1111/j.1432-1033.1968.tb00398.x.
6
Structural organization of nuclear complexes containing DNA-like RNA.含有类DNA RNA的核复合物的结构组织
J Mol Biol. 1968 Apr 14;33(1):251-63. doi: 10.1016/0022-2836(68)90292-1.
7
Isoelectric points and conformation of proteins. I. Effect of urea on the behavior of some proteins in isoelectric focusing.蛋白质的等电点和构象。I. 尿素对某些蛋白质在等电聚焦中行为的影响。
Biochim Biophys Acta. 1971 Mar 23;229(3):567-81.
8
Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication.在腺病毒复制过程中，向病毒特异性RNA添加聚腺苷酸序列。
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2806-9. doi: 10.1073/pnas.68.11.2806.
9
Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship.海拉细胞异质性核RNA和快速标记的多核糖体RNA中的聚腺苷酸序列：前体关系的可能证据。
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1336-40. doi: 10.1073/pnas.68.6.1336.
10
A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells.在小鼠肉瘤180腹水细胞快速标记的多核糖体RNA组分中富含腺苷酸的多核苷酸片段。
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1331-5. doi: 10.1073/pnas.68.6.1331.

通过聚腺苷酸-琼脂糖亲和层析从人宫颈癌（HeLa）细胞胞质中分离出的一种蛋白质的特性分析。

Characterization of a protein species isolated from HeLa cell cytoplasm by affinity chromatography on polyadenylate-sepharose.

作者信息

Blanchard J M, Brissac C, Jeanteur P

出版信息

Proc Natl Acad Sci U S A. 1974 May;71(5):1882-6. doi: 10.1073/pnas.71.5.1882.

DOI:10.1073/pnas.71.5.1882
PMID:4525299
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC388346/
Abstract

Chromatography of different soluble extracts from HeLa cells on poly(A)-Sepharose columns has allowed the isolation of a protein fraction eluted by 0.2 M NaCl and localized predominantly in the cytoplasmic supernatant and in the 0.5 M KCl ribosomal wash. This fraction is present in large amounts (around 3% of total cytosolic proteins) and appears to contain a major protein species that is acidic on electrofocusing (pI around 4.5) and phosphorylated. It runs on glycerol gradients and Sephadex G-200 chromatography close to the aldolase marker (158,000 daltons) and dissociates into apparently identical subunits of 38,000 +/- 2,000 daltons on sodium dodecyl sulfate-acrylamide gels, suggesting a tetrameric structure.

摘要

对来自HeLa细胞的不同可溶性提取物在聚（A）-琼脂糖柱上进行色谱分析，已分离出一个蛋白质组分，该组分用0.2M NaCl洗脱，主要定位于细胞质上清液和0.5M KCl核糖体洗脱液中。该组分含量丰富（约占总胞质蛋白的3%），似乎含有一种主要蛋白质，在等电聚焦时呈酸性（pI约为4.5）且被磷酸化。它在甘油梯度和Sephadex G - 200色谱上的行为与醛缩酶标记物（158,000道尔顿）相近，在十二烷基硫酸钠 - 丙烯酰胺凝胶上解离为明显相同的38,000 +/- 2,000道尔顿的亚基，提示其为四聚体结构。