Lang D R, Felix J, Lundgren D G
J Bacteriol. 1972 Jun;110(3):968-77. doi: 10.1128/jb.110.3.968-977.1972.
Bulk membrane fragments were prepared from cells of Bacillus cereus ATCC 4342 harvested at different stages of growth and sporulation and examined for enzymes involved in electron transport functions. The presence of succinate: DCPIP oxidoreductase (EC 1.3.99.1), succinate: cytochrome c oxidoreductase (EC 1.3.2.1), NADH:DCPIP oxidoreductase (EC 1.6.99.1), NADH:cytochrome c oxidoreductase (EC 1.6.2.1), succinate oxidase [succinate: (O(2)) oxidoreductase, EC 1.3.3.1], and NADH oxidase [NADH:(O(2)) oxidoreductase, EC 1.6.3.1] were demonstrated in membrane fragments from vegetative cells, early and late stationary-phase cells, and in cells undergoing sporulation. During the transition from a vegetative cell to a spore, there was a significant increase in the levels of enzymes associated with energy production via the electron transport system. Cytochromes of the a, b, and c type were detected in all membrane preparations; however, there was a marked increase in the level of cytochromes by the end of vegetative growth which remained throughout sporulation; there were no qualitative changes in the cytochromes throughout growth and sporulation. Sporulation was inhibited by cyanide, stressing the significance of the electron transport system. Enzyme activities were partially masked in washed membrane fragments; however, unmasking (stimulation) was achieved by sodium deoxycholate, sodium dodecyl sulfate, or Triton X-100. The degree of enzyme masking was less in vegetative cell membrane fragments than in membranes prepared from stationary-phase or sporulating cells. Results indicate the development of a membrane-bound electron transport system in B. cereus by the end of growth and prior to sporulation, which results in an increased masking of a number of enzymes associated with the terminal respiratory system of the cell.
从不同生长阶段和孢子形成阶段收获的蜡样芽孢杆菌ATCC 4342细胞制备了大量膜碎片，并检测了参与电子传递功能的酶。在营养细胞、早期和晚期稳定期细胞以及正在进行孢子形成的细胞的膜碎片中均证实存在琥珀酸：DCPIP氧化还原酶（EC 1.3.99.1）、琥珀酸：细胞色素c氧化还原酶（EC 1.3.2.1）、NADH：DCPIP氧化还原酶（EC 1.6.99.1）、NADH：细胞色素c氧化还原酶（EC 1.6.2.1）、琥珀酸氧化酶[琥珀酸：（O₂）氧化还原酶，EC 1.3.3.1]和NADH氧化酶[NADH：（O₂）氧化还原酶，EC 1.6.3.1]。在从营养细胞向孢子转变过程中，通过电子传递系统与能量产生相关的酶水平显著增加。在所有膜制剂中均检测到a、b和c型细胞色素；然而，在营养生长结束时细胞色素水平显著增加，并在整个孢子形成过程中保持；在整个生长和孢子形成过程中细胞色素没有定性变化。氰化物抑制孢子形成，强调了电子传递系统的重要性。酶活性在洗涤后的膜碎片中部分被掩盖；然而，通过脱氧胆酸钠、十二烷基硫酸钠或 Triton X-100可实现去掩盖（刺激）。营养细胞膜碎片中酶的掩盖程度低于稳定期或孢子形成细胞制备的膜。结果表明，在生长结束和孢子形成之前，蜡样芽孢杆菌中形成了膜结合电子传递系统，这导致与细胞末端呼吸系统相关的多种酶的掩盖增加。
