猴病毒40的遗传分析。IV. 一种温度敏感型早期突变体对Balb-3T3细胞转化的抑制作用
Genetic analysis of simian virus 40. IV. Inhibited transformation of Balb-3T3 cells by a temperature-sensitive early mutant.
作者信息
Robb J A, Smith H S, Scher C D
出版信息
J Virol. 1972 Jun;9(6):969-72. doi: 10.1128/JVI.9.6.969-972.1972.
Abstract
The temperature-sensitive early mutant, ts(*)101, was characterized during productive infection in monkey cells, and the results are presented in an accompanying paper. This paper demonstrates that although 101 mutant virions adsorb normally to confluent Balb/3T3 mouse cells at both permissive (33 C) and restrictive (38.5 C) temperatures, T antigen synthesis and transformation, abortive and stable, are inhibited at both temperatures (host-range inhibition). T antigen synthesis is temperature sensitive, whereas abortive and stable transformation are not. Clones of 101-transformed Balb/3T3 cells were isolated, and virus was rescued from all clones at both permissive and restrictive temperatures. The rescued virus was as temperature sensitive as the original transforming 101 virions.
摘要
温度敏感早期突变体ts(*)101在猴细胞的增殖性感染过程中得到了鉴定，相关结果发表在一篇随附论文中。本文表明，尽管101突变体病毒粒子在允许温度（33℃）和限制温度（38.5℃）下均能正常吸附至汇合的Balb/3T3小鼠细胞，但在这两种温度下，T抗原的合成以及流产型和稳定型转化均受到抑制（宿主范围抑制）。T抗原的合成对温度敏感，而流产型和稳定型转化则不然。分离出了101转化的Balb/3T3细胞克隆，在允许温度和限制温度下均从所有克隆中拯救出了病毒。拯救出的病毒与原始转化性101病毒粒子一样对温度敏感。
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Genetic analysis of simian virus 40. 3. Characterization of a temperature-sensitive mutant blocked at an early stage of productive infection in monkey cells.猴病毒40的遗传分析。3. 一种在猴细胞生产性感染早期受阻的温度敏感突变体的特性。
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