Vengris V E, Maré C J
Can J Comp Med. 1972 Jul;36(3):282-7.
The production of interferon by porcine kidney (PK(15)) cell culture in response to viral and synthetic inducers was studied. The inducers used included a synthetic double-stranded polyribonucleotide, polyriboinosinic-polyribocytidylic acid (Poly I:C), swine influenza virus and three strains of pseudorabies virus. Following exposure to these inducers cell culture fluids were examined for interferon by the plaque-reduction method. The Poly I:C and the swine influenza virus induced production of interferon by PK(15) cell cultures, whereas, all three strains of pseudorabies virus at the two concentrations tested failed to induce production of interferon in vitro. The antiviral substance produced in PK(15) cells was identified as an interferon because it was pH stable, non-dialyzable, sensitive to trypsin, non-sedimentable, relatively heat stable, host-species specific and it possessed broad-spectrum antiviral activity. The latter was demonstrated by inhibition of vesicular stomatitis, vaccinia and pseudorabies viruses. Differences in interferon activity against the different viruses were observed.
研究了猪肾（PK(15)）细胞培养物对病毒和合成诱导剂产生干扰素的情况。所用诱导剂包括合成双链多聚核糖核苷酸聚肌苷酸-聚胞苷酸（Poly I:C）、猪流感病毒和三株伪狂犬病病毒。在接触这些诱导剂后，通过蚀斑减少法检测细胞培养液中的干扰素。Poly I:C和猪流感病毒可诱导PK(15)细胞培养物产生干扰素，而在所测试的两种浓度下，所有三株伪狂犬病病毒均未能在体外诱导干扰素的产生。PK(15)细胞产生的抗病毒物质被鉴定为干扰素，因为它对pH稳定、不可透析、对胰蛋白酶敏感、不可沉淀、相对耐热、具有宿主物种特异性且具有广谱抗病毒活性。后者通过对水疱性口炎病毒、痘苗病毒和伪狂犬病病毒的抑制作用得以证明。观察到针对不同病毒的干扰素活性存在差异。
