Chaudhary R K, Westwood J C
Appl Microbiol. 1972 Aug;24(2):270-4. doi: 10.1128/am.24.2.270-274.1972.
Insoluble polyelectrolytes (PE60) were used for the concentration of viruses from stool specimens, confirming the results of Wallis et al. (1969). Ten percent suspensions inoculated with poliovirus type 3 were used in these experiments. A small number of stool specimens from patients naturally infected with enteroviruses were also tested. Preferential adsorption of viruses to PE60 was maximum at a pH range of 4.5 to 6.0. The elution of the adsorbed viruses was optimal at pH 8.5. Other parameters were also investigated. Electron microscopy was used successfully to detect the eluted viruses.
使用不溶性聚电解质（PE60）从粪便标本中浓缩病毒，证实了沃利斯等人（1969年）的结果。这些实验中使用了接种3型脊髓灰质炎病毒的10%悬液。还检测了少量自然感染肠道病毒患者的粪便标本。病毒对PE60的优先吸附在pH值4.5至6.0范围内最大。吸附病毒的洗脱在pH值8.5时最佳。还研究了其他参数。成功使用电子显微镜检测洗脱的病毒。
