Sorber C A, Sagik B P, Malina J F
Appl Microbiol. 1971 Sep;22(3):334-8. doi: 10.1128/am.22.3.334-338.1971.
The insoluble polyelectrolyte technique for concentrating virus is extended to extremely low virus levels. The effectiveness of this method employing a coliphage T2 model is a constant 20% over a range of virus levels from 10(3) to 10(-4) plaque-forming units/ml. The efficiency of the method is dependent upon pH control during the concentration phase. Although the study was initiated to develop a method for quantitating the effectiveness of water and wastewater treatment methods for the removal of viruses from waters at low concentrations, the potential of the technique for efficient monitoring of natural waters is apparent.
用于浓缩病毒的不溶性聚电解质技术被扩展到极低的病毒水平。采用大肠杆菌噬菌体T2模型的这种方法在病毒水平从10³到10⁻⁴ 噬菌斑形成单位/毫升的范围内,有效性始终为20%。该方法的效率取决于浓缩阶段的pH控制。尽管开展这项研究是为了开发一种定量水和废水处理方法去除水中低浓度病毒有效性的方法,但该技术用于高效监测天然水的潜力是显而易见的。