从污水和排泄物中通过不溶性聚电解质浓缩病毒。
Concentration of viruses from sewage and excreta on insoluble polyelectrolytes.
作者信息
Wallis C, Grinstein S, Melnick J L, Fields J E
出版信息
Appl Microbiol. 1969 Dec;18(6):1007-14. doi: 10.1128/am.18.6.1007-1014.1969.
Abstract
The concentration of viruses from sewage by adsorption on and elution from an insoluble cross-linked copolymer of maleic anhydride is described. Viruses either added to sewage or naturally contained in sewage were preferentially adsorbed to this polyelectrolyte at a pH range of 5.0 to 6.0 and were eluted at pH 8.0 to 9.0. In a 2-month survey of viruses in sewage in the spring (April to May 1968), when viruses are at low levels, efficient and economical detection of these agents was accomplished with the polyelectrolyte-concentration method. This method lends itself to the detection of viruses present in minute amounts in fecal samples, urine, sewage, and other natural waters. Large volumes of these fluids can be treated with the polymer described, and virus can be concentrated sufficiently for detection.
摘要
本文描述了通过吸附在不溶性马来酸酐交联共聚物上并从其上洗脱来浓缩污水中病毒的方法。添加到污水中或天然存在于污水中的病毒在pH值为5.0至6.0的范围内优先吸附到这种聚电解质上，并在pH值为8.0至9.0时被洗脱。在1968年春季（4月至5月）对污水中病毒进行的为期2个月的调查中，当病毒含量较低时，采用聚电解质浓缩法可有效且经济地检测这些病原体。该方法适用于检测粪便样本、尿液、污水及其他天然水体中微量存在的病毒。可以用所述聚合物处理大量的这些液体，并且可以充分浓缩病毒以进行检测。
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