从蓝蟹（Callinectes sapidus）中分离轮状病毒和肠道病毒
Concentration of rotavirus and enteroviruses from blue crabs (Callinectes sapidus).
作者信息
Seidel K M, Goyal S M, Rao V C, Melnick J L
出版信息
Appl Environ Microbiol. 1983 Dec;46(6):1293-6. doi: 10.1128/aem.46.6.1293-1296.1983.
Abstract
A simple method for concentration and detection of rotavirus and enteroviruses in the blue crab is described. Virus was separated from tissue homogenates at pH 9.5, concentrated by adsorption to protein precipitates at pH 3.5, and recovered by elution of precipitates at pH 9.2. Test samples of 12 to 15 ml were produced from an initial 100 g of crab tissues. Cat-floc precipitation was used to remove sample toxicity for cell cultures. Recovery effectiveness averaged 52% with poliovirus 1, echovirus 7, and coxsackievirus B5 and 23% with rotavirus SA11.
摘要
本文描述了一种用于浓缩和检测蓝蟹中轮状病毒和肠道病毒的简单方法。病毒在pH 9.5条件下从组织匀浆中分离出来，通过在pH 3.5条件下吸附到蛋白质沉淀上进行浓缩，并在pH 9.2条件下洗脱沉淀来回收。最初100克蟹组织可制备出12至15毫升的测试样品。采用猫絮凝沉淀法去除样品对细胞培养的毒性。脊髓灰质炎病毒1型、埃可病毒7型和柯萨奇病毒B5的回收效率平均为52%，轮状病毒SA11的回收效率为23%。
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