用于诊断血清学的简易放射免疫测定法。
Simplified radioimmunoassay for diagnostic serology.
作者信息
Hutchinson H D, Ziegler D W
出版信息
Appl Microbiol. 1972 Nov;24(5):742-9. doi: 10.1128/am.24.5.742-749.1972.
Abstract
A simplified, indirect radioimmunoassay is described for Escherichia coli, vaccinia virus, and herpesvirus. The antigens were affixed to glass cover slips; thus both the primary and secondary reactions take place on the cover slips, and the unbound antiserum is easily separated from the bound antiserum by rinsing. Rabbit or human immune sera were reacted with the antigens, and the primary immune complex was quantitated by a secondary reaction with (125)I-indicator globulin (anti-rabbit or anti-human). A direct relationship between the antiserum concentration and the (125)I absorption was established. Variations in titers were detectable, and the titers were comparable to complement fixation titers. Homologous and heterologous reactions were distinguishable. The method affords an objective, quantitative, and qualitative evaluation of antibody, and results are reproducible.
摘要
本文描述了一种用于检测大肠杆菌、痘苗病毒和疱疹病毒的简化间接放射免疫测定法。抗原固定在玻璃盖玻片上；因此，一级反应和二级反应均在盖玻片上进行，通过冲洗可轻松将未结合的抗血清与结合的抗血清分离。兔或人免疫血清与抗原反应，通过与（125）I标记球蛋白（抗兔或抗人）的二级反应对一级免疫复合物进行定量。确定了抗血清浓度与（125）I吸收之间的直接关系。可检测到滴度的变化，且这些滴度与补体结合滴度相当。同源反应和异源反应可区分。该方法可对抗体进行客观、定量和定性评估，结果具有可重复性。
相似文献
1
Simplified radioimmunoassay for diagnostic serology.用于诊断血清学的简易放射免疫测定法。
Appl Microbiol. 1972 Nov;24(5):742-9. doi: 10.1128/am.24.5.742-749.1972.
2
Comparison of direct and indirect solid-phase microradioimmunoassays for the detection of viral antigens and antiviral antibody.用于检测病毒抗原和抗病毒抗体的直接和间接固相微放射免疫测定法的比较。
Appl Microbiol. 1973 Apr;25(4):567-73. doi: 10.1128/am.25.4.567-573.1973.
3
Rapid micro-radioimmunoassay for the measurement of antiviral antibody.用于测量抗病毒抗体的快速微量放射免疫测定法。
J Immunol. 1972 Jul;109(1):171-3.
4
Virus antigens on the surface of infected cells: binding and elution of ( 125 I)-labelled antivirus antibody.受感染细胞表面的病毒抗原：（125I）标记抗病毒抗体的结合与洗脱
J Gen Virol. 1973 Feb;18(2):195-9. doi: 10.1099/0022-1317-18-2-195.
5
Radioimmunoassay techniques for detecting naturally occurring viral antibody in human sera.用于检测人血清中天然存在的病毒抗体的放射免疫分析技术。
J Immunol Methods. 1974 Oct;5(4):337-44. doi: 10.1016/0022-1759(74)90017-9.
6
Interaction of staphylococcal protein A with virus-IgG complexes.葡萄球菌蛋白A与病毒-IgG复合物的相互作用。
J Immunol. 1974 Nov;113(5):1568-74.
7
Increase in preexisting cellular antigen-combining groups at different times after infection with different viruses.感染不同病毒后不同时间原有细胞抗原结合基团的增加。
Proc Natl Acad Sci U S A. 1970 Oct;67(2):731-7. doi: 10.1073/pnas.67.2.731.
8
Sensitivity of a radioimmunoassay method for detection of certain viral antibodies in sera and cerebrospinal fluids.一种用于检测血清和脑脊液中某些病毒抗体的放射免疫测定方法的灵敏度。
J Clin Microbiol. 1976 Dec;4(6):470-8. doi: 10.1128/jcm.4.6.470-478.1976.
9
Detection by radioimmunoassay of antibodies in human smallpox patients and vaccinees.通过放射免疫分析法检测天花患者和接种疫苗者体内的抗体。
J Clin Microbiol. 1975 Mar;1(3):311-7. doi: 10.1128/jcm.1.3.311-317.1975.
10
Identification of measles virus-specific hemolysis-inihibiting antibodies separate from hemagglutination-inhibiting antibodies.鉴定与血凝抑制抗体分离的麻疹病毒特异性溶血抑制抗体。
Infect Immun. 1975 Feb;11(2):231-9. doi: 10.1128/iai.11.2.231-239.1975.
引用本文的文献
1
Review of bovine immunology for the veterinary practitioner.兽医从业者的牛免疫学综述。
Can Vet J. 1974 Sep;15(9):243-8.
2
Radioimmunoassay on polycarbonate membranes: a sensitive and simplified method for the detection and quantitation of antibody.聚碳酸酯膜上的放射免疫测定：一种用于抗体检测和定量的灵敏且简化的方法。
Appl Microbiol. 1974 Mar;27(3):475-9. doi: 10.1128/am.27.3.475-479.1974.
3
Criteria for preparing, evaluating, and standardizing iodinated globulins for radioimmunoassay procedures.放射免疫分析程序中碘化球蛋白的制备、评估及标准化标准。
Appl Microbiol. 1974 Dec;28(6):935-42. doi: 10.1128/am.28.6.935-942.1974.
4
Screening for mumps immunity with the microtiter solid-phase radioimmunoassay.采用微量滴定固相放射免疫测定法筛查腮腺炎免疫力。
Infection. 1976;4(3):121-4. doi: 10.1007/BF01638936.
5
Solid-phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies.风疹病毒免疫球蛋白G和免疫球蛋白M抗体的固相放射免疫测定
J Clin Microbiol. 1976 Aug;4(2):117-23. doi: 10.1128/jcm.4.2.117-123.1976.
6
Local antibody response in chickens: analysis of antibody synthesis to Newcastle disease virus by solid-phase radioimmunoassay and immunofluorescence with class-specific antibody for chicken immunoglobulins.鸡的局部抗体反应：通过固相放射免疫测定法以及使用针对鸡免疫球蛋白的类特异性抗体进行免疫荧光分析，检测针对新城疫病毒的抗体合成情况。
Infect Immun. 1979 Apr;24(1):269-75. doi: 10.1128/iai.24.1.269-275.1979.
7
Microassay for immunoglobulin G antibodies to Treponema pallidum with radioiodinated protein A from staphylococcus aureus: immunoglobulin G response in experimental syphilis in rabbits.用金黄色葡萄球菌放射性碘化蛋白A进行梅毒螺旋体免疫球蛋白G抗体的微量测定：兔实验性梅毒中的免疫球蛋白G反应
Infect Immun. 1978 Jul;21(1):163-70. doi: 10.1128/iai.21.1.163-170.1978.
8
Comparison of rabies humoral antibody titers in rabbits and humans by indirect radioimmunoassay, rapid-fluorescent-focus-inhibition technique, and indirect fluorescent-antibody assay.通过间接放射免疫测定法、快速荧光灶抑制技术和间接荧光抗体测定法比较兔和人狂犬病体液抗体滴度。
J Clin Microbiol. 1977 Mar;5(3):320-5. doi: 10.1128/jcm.5.3.320-325.1977.
9
Sensitivity of a radioimmunoassay method for detection of certain viral antibodies in sera and cerebrospinal fluids.一种用于检测血清和脑脊液中某些病毒抗体的放射免疫测定方法的灵敏度。
J Clin Microbiol. 1976 Dec;4(6):470-8. doi: 10.1128/jcm.4.6.470-478.1976.
10
Detection of virus-specific immunoglobulins using a doubly labeled fluorescein-125I antibody.使用双标记荧光素-125I抗体检测病毒特异性免疫球蛋白。
J Clin Microbiol. 1976 Jun;3(6):637-9. doi: 10.1128/jcm.3.6.637-639.1976.
本文引用的文献
1
The serum proteins in multiple myelomatosis.多发性骨髓瘤中的血清蛋白
Biochem J. 1940 Sep;34(8-9):1248-57. doi: 10.1042/bj0341248.
2
THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.高比放射性碘-131标记人生长激素的制备
Biochem J. 1963 Oct;89(1):114-23. doi: 10.1042/bj0890114.
3
Precipitation of radiolabeled poliovirus with specific antibody and antiglobulin.用特异性抗体和抗球蛋白沉淀放射性标记的脊髓灰质炎病毒。
J Immunol. 1962 Oct;89:559-70.
4
Frequency of antibody to hepatitis-associated antigen as measured by a new radioimmunoassay technique.采用一种新的放射免疫测定技术所测得的肝炎相关抗原抗体频率。
J Immunol. 1971 May;106(5):1166-71.
5
An approach toward automation of antinuclear antibody (ANA) determination.一种抗核抗体（ANA）检测自动化的方法。
Ann N Y Acad Sci. 1971 Jun 21;177:459-66. doi: 10.1111/j.1749-6632.1971.tb35076.x.
6
Automation of an indirect fluorescent antibody test for syphilis.梅毒间接荧光抗体试验的自动化
Ann N Y Acad Sci. 1971 Jun 21;177:453-8. doi: 10.1111/j.1749-6632.1971.tb35075.x.
7
A semiautomatic procedure for routine screening by immunofluorescence techniques.一种通过免疫荧光技术进行常规筛查的半自动程序。
Ann N Y Acad Sci. 1971 Jun 21;177:446-52. doi: 10.1111/j.1749-6632.1971.tb35074.x.
8
General principles of radioimmunoassay.放射免疫分析的一般原理。
Clin Chim Acta. 1968 Sep;22(1):51-69. doi: 10.1016/0009-8981(68)90247-7.
9
10
Application of the paired label radioantibody technique to tissue sections and cell smears.配对标记放射抗体技术在组织切片和细胞涂片上的应用。
J Immunol. 1967 Feb;98(2):274-80.
Zotero 插件