放射免疫分析程序中碘化球蛋白的制备、评估及标准化标准。
Criteria for preparing, evaluating, and standardizing iodinated globulins for radioimmunoassay procedures.
作者信息
Hutchinson H D, Ziegler D W
出版信息
Appl Microbiol. 1974 Dec;28(6):935-42. doi: 10.1128/am.28.6.935-942.1974.
Abstract
Procedures were examined for labeling immune globulins with radioactive iodine using chloramine-T as the oxidizing agent. The chloramine-T method was critically evaluated to establish the optimal conditions for preparing iodinated globulins with high specific radioactivities without impairing their immunospecificities for use in in vitro radioimmunoassays. The results showed that the use of 100 mug of chloramine-T per ml, 500 to 1,000 muCi of Na (125)I per mg of protein, and a 10-min oxidation reaction time produced globulins of both high specific radioactivities and immunospecificities. Criteria were established for evaluating and determining optimal concentrations of iodine-labeled globulin for use in radioimmunoassays. The results of this investigation indicated that the amount of labeled indicator globulin used in radioimmunoassays should be based upon protein concentration rather than radioactivity.
摘要
研究了以氯胺 - T作为氧化剂用放射性碘标记免疫球蛋白的方法。对氯胺 - T法进行了严格评估，以确定在不损害其免疫特异性的情况下制备具有高比放射性的碘化球蛋白用于体外放射免疫分析的最佳条件。结果表明，每毫升使用100微克氯胺 - T、每毫克蛋白质使用500至1000微居里的Na(125)I以及10分钟的氧化反应时间，可产生具有高比放射性和免疫特异性的球蛋白。建立了评估和确定用于放射免疫分析的碘标记球蛋白最佳浓度的标准。这项研究的结果表明，放射免疫分析中使用的标记指示球蛋白的量应基于蛋白质浓度而非放射性。
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