大鼠肝脏中醛脱氢酶的细胞内定位
Intracellular localization of aldehyde dehydrogenase in rat liver.
作者信息
Marjanen L
出版信息
Biochem J. 1972 May;127(4):633-9. doi: 10.1042/bj1270633.
Abstract
- Distribution of aldehyde dehydrogenase activity in rat liver was studied by measuring the rate of disappearance of acetaldehyde in the presence of each of the subcellular fractions. These were obtained by rough separation of particulate fractions from the soluble portion of the cell, by differential centrifugation, and by isopycnic gradient centrifugation. 2. The maximal rate of acetaldehyde oxidation was 3.7 mumol/min per g, with an apparent K(m) value below 10(-5)m. The highest rate of activity was observed in phosphate buffers of high P(i) concentration (above 60mm). 3. The activity measured was completely dependent on NAD(+). 4. The microsomal fraction and the nuclei were inactive in the assay. Of the total activity 80% was found in the mitochondrial fraction and the remaining 20% in the cytoplasm. 5. The distribution pattern is important from the point of view of acetaldehyde oxidation during ethanol metabolism. The apparent discrepancy of the results obtained by different workers and the localization of acetaldehyde oxidation in vivo is discussed.
摘要
- 通过测量在各亚细胞组分存在的情况下乙醛消失的速率，研究了大鼠肝脏中乙醛脱氢酶活性的分布。这些亚细胞组分是通过差速离心和等密度梯度离心，从细胞的可溶部分粗分离颗粒组分而获得的。2. 乙醛氧化的最大速率为每克3.7微摩尔/分钟，表观K(m)值低于10(-5)m。在高P(i)浓度（高于60毫米）的磷酸盐缓冲液中观察到最高活性速率。3. 测得的活性完全依赖于NAD(+)。4. 在该测定中，微粒体组分和细胞核无活性。在总活性中，80%存在于线粒体组分中，其余20%存在于细胞质中。5. 从乙醇代谢过程中乙醛氧化的角度来看，这种分布模式很重要。讨论了不同研究者所得结果的明显差异以及体内乙醛氧化的定位。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
A microspectrophotometric method for the determination of cytochrome oxidase.一种测定细胞色素氧化酶的显微分光光度法。
J Biol Chem. 1951 Apr;189(2):665-70.
3
Hepatic aldehyde oxidase. I. Purification and properties.肝醛氧化酶。I. 纯化与性质
J Biol Chem. 1962 Mar;237:922-8.
4
The relation between intra- and extramitochondrial pyridine nucleotides.线粒体内外吡啶核苷酸之间的关系。
J Biol Chem. 1961 Oct;236:2794-803.
5
The metabolism of acetaldehyde in mammalian tissues. Reactions in rat-liver suspensions under aerobic conditions.哺乳动物组织中乙醛的代谢。有氧条件下大鼠肝脏悬浮液中的反应。
Biochem J. 1962 Aug;84(2):281-6. doi: 10.1042/bj0840281.
6
The metabolism of acetaldehyde in mammalian tissues: reactions in rat-liver suspensions under anaerobic conditions.乙醛在哺乳动物组织中的代谢：厌氧条件下大鼠肝脏悬浮液中的反应。
Biochem J. 1959 Jul;72(3):409-19. doi: 10.1042/bj0720409.
7
COUPLED ALDEHYDE DEHYDROGENASE AND CATALASE IN MITOCHONDRIAL EXTRACTS.
Arch Biochem Biophys. 1965 Apr;110:104-16. doi: 10.1016/0003-9861(65)90160-8.
8
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J Cell Biol. 1964 Aug;22(2):317-26. doi: 10.1083/jcb.22.2.317.
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