未感染及劳斯肉瘤病毒转化的禽细胞的膜蛋白。
Membrane proteins of uninfected and Rous sarcoma virus- transformed avian cells.
作者信息
Bussell R H, Robinson W S
出版信息
J Virol. 1973 Aug;12(2):320-7. doi: 10.1128/JVI.12.2.320-327.1973.
Abstract
A method for preparing large membrane fragments and cell ghosts was developed for uninfected and Rous sarcoma virus-transformed chicken embryo fibroblasts in culture. Membrane proteins were analyzed by electrophoresis in acrylamide gels containing sodium dodecyl sulfate. A major amino-acid-containing component of uninfected cell membranes was greatly diminished in amount or absent in membranes of virus-transformed cells. This component, called MP-1, had an electrophoretic mobility in sodium dodecyl sulfate-containing gels similar to that of a protein of a mol wt of 1.42 x 10(5). MP-1 was not altered by changes in cell growth rate or in cells infected with the nontransforming virus RAV-1.
摘要
已开发出一种制备大膜片段和细胞空壳的方法，用于培养中的未感染及劳斯肉瘤病毒转化的鸡胚成纤维细胞。通过在含有十二烷基硫酸钠的丙烯酰胺凝胶中进行电泳来分析膜蛋白。未感染细胞膜中一种主要的含氨基酸成分在病毒转化细胞的膜中含量大幅减少或缺失。这种成分称为MP-1，在含十二烷基硫酸钠的凝胶中的电泳迁移率与分子量为1.42×10⁵的一种蛋白质相似。MP-1不会因细胞生长速率的变化或感染非转化病毒RAV-1的细胞而改变。
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