Hegenauer J, Saltman P, Hatlen L
Biochem J. 1979 Feb 1;177(2):693-5. doi: 10.1042/bj1770693.
The cadmium content of crystallized horse spleen ferritin, usually about 2% by weight without special treatment, can be substantially decreased by prolonged dialysis against certain chelating agents, chaotropic ions, or weakly reducing anions. For example, neutral bisulphite buffer (2M) removed 95% of the bound cadmium of crystallization without affecting the iron content, and may thus be valuable for preparing "metal-free" holoferritin for physical-chemical studies.
结晶马脾铁蛋白的镉含量在未经特殊处理时通常约为2%（重量），通过用某些螯合剂、离液序列高的离子或弱还原性阴离子进行长时间透析，其镉含量可大幅降低。例如，中性亚硫酸氢盐缓冲液（2M）可去除95%的结晶结合镉，而不影响铁含量，因此对于制备用于物理化学研究的“无金属”全铁蛋白可能很有价值。
