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干扰素：其糖蛋白性质的证据。

Interferon: evidence for its glycoprotein nature.

作者信息

Dorner F, Scriba M, Weil R

出版信息

Proc Natl Acad Sci U S A. 1973 Jul;70(7):1981-5. doi: 10.1073/pnas.70.7.1981.

DOI:10.1073/pnas.70.7.1981
PMID:4352965
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC433647/
Abstract

In an attempt to understand the structure of rabbit interferon, the possibility of carbohydrate being part of the molecule was tested. Interferon incubated with neuraminidase from Vibrio cholera is homogeneous in charge as revealed by isoelectric focusing. Treatment of "asialointerferon" with galactose oxidase (EC 1.1.3.9) from Dactylium dendroides and subsequent reduction with tritiated sodium borohydride yields labeled material with unimpaired antiviral activity. Enzymic incorporation of N-[(14)C]acetylneuraminic acid into tritiated asialointerferon restores the original charge heterogeneity. The newly generated sialointerferon contains both (3)H and (14)C activity. Asialointerferon is retained by an affinity column containing phytohemagglutinin from Phaseolus vulgaris and can be displaced from the adsorbent by a glycoprotein of known structure. It is concluded that rabbit interferon is a glycoprotein containing the terminal oligosaccharide sequence sialic acid --> galactose.

摘要

为了了解兔干扰素的结构，对碳水化合物作为该分子一部分的可能性进行了测试。经等电聚焦显示，用霍乱弧菌神经氨酸酶孵育的干扰素在电荷方面是均匀的。用来自树状指霉的半乳糖氧化酶（EC 1.1.3.9）处理“去唾液酸干扰素”，随后用氚化硼氢化钠还原，产生具有未受损抗病毒活性的标记物质。将N-[(14)C]乙酰神经氨酸酶促掺入氚化去唾液酸干扰素中可恢复原始的电荷异质性。新产生的唾液酸干扰素同时含有(3)H和(14)C活性。去唾液酸干扰素被含有来自菜豆的植物血凝素的亲和柱保留，并且可以被已知结构的糖蛋白从吸附剂上置换下来。得出的结论是，兔干扰素是一种含有末端寡糖序列唾液酸→半乳糖的糖蛋白。

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本文引用的文献

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6
Interferon: evidence for subunit structure.干扰素：亚基结构的证据。
Proc Natl Acad Sci U S A. 1970 Oct;67(2):620-8. doi: 10.1073/pnas.67.2.620.
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Regulation of cellular interferon production: enhancement by antimetabolites.细胞干扰素产生的调节：抗代谢物的增强作用。
Proc Natl Acad Sci U S A. 1970 Sep;67(1):464-71. doi: 10.1073/pnas.67.1.464.
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Solubilization and partial characterization of a phytohemagglutinin receptor site from human erythrocytes.人红细胞中植物血凝素受体位点的增溶及部分特性分析
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Adsorbents for affinity chromatography. Use of N-hydroxysuccinimide esters of agarose.亲和层析吸附剂。琼脂糖N-羟基琥珀酰亚胺酯的应用。
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The action of proteolytic enzymes on N,N-dimethyl proteins. Basis for a microassay for proteolytic enzymes.蛋白水解酶对N，N - 二甲基蛋白质的作用。一种蛋白水解酶微量测定法的基础。
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