帕吉林对猫脾脏中递质释放及去甲肾上腺素摄取的影响。

Twenty minutes after the addition of pargyline (5 x 10(-4)M) to blood perfusing the isolated spleen of the cat, the overflow of transmitter resulting from stimulation of the sympathetic nerves increased 2.3-3-fold. Lower doses of pargyline did not significantly affect overflow.2. Monoamine oxidase activity, measured with either radioactively labelled tyramine or noradrenaline as substrate, was almost completely inhibited by doses of pargyline in the range of 10(-4)M to 5 x 10(-4)M. Inhibition of enzyme activity was not correlated with the effect on overflow. Pargyline had only a slight inhibitory effect on catechol-O-methyl transferase.3. Uptake of a 1 mug injection (pulse) of labelled noradrenaline, following pargyline (5 x 10(-4)M), was increased to 199.1% of that found in control experiments.4. Pargyline significantly reduced the vascular responses to nerve stimulation but had no significant effect on capsular responses.5. The inhibitor had no effect on resting overflow of labelled noradrenaline from the spleen but doubled the overflow of labelled noradrenaline following nerve stimulation.6. It is suggested that the effect of pargyline on overflow is due to increased release of transmitter during nerve stimulation.7. The possible clinical significance of these findings is discussed.

向灌注猫离体脾脏的血液中加入优降宁（5×10⁻⁴M）20分钟后，交感神经受刺激导致的递质溢出增加了2.3至3倍。较低剂量的优降宁对溢出无显著影响。
以放射性标记的酪胺或去甲肾上腺素为底物测量的单胺氧化酶活性，在10⁻⁴M至5×10⁻⁴M范围内的优降宁剂量几乎完全被抑制。酶活性的抑制与对溢出的影响无关。优降宁对儿茶酚-O-甲基转移酶只有轻微的抑制作用。
加入优降宁（5×10⁻⁴M）后，1微克注射（脉冲）标记去甲肾上腺素的摄取增加到对照实验中发现值的199.1%。
优降宁显著降低了对神经刺激的血管反应，但对包膜反应无显著影响。
该抑制剂对脾脏中标记去甲肾上腺素的静息溢出无影响，但使神经刺激后标记去甲肾上腺素的溢出增加了一倍。
提示优降宁对溢出的影响是由于神经刺激期间递质释放增加。
讨论了这些发现可能的临床意义。

The effects of pargyline on overflow of transmitter and uptake of noradrenaline in the cat spleen.