一种可通过温和蛋白酶解从哺乳动物细胞中去除的“胞外”RNA。
An "external" RNA removable from mammalian cells by mild proteolysis.
作者信息
Rieber M, Bacalao J
出版信息
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4960-4. doi: 10.1073/pnas.71.12.4960.
Brief exposure of Chinese hamster ovary cell monolayers prelabeled with [(32)P]phosphate and [(3)H]leucine to 1 mug/ml of trypsin under conditions in which cells remain fully viable causes the release of macromolecular (32)P and (3)H. Whereas ribonuclease treatment was found to affect markedly both the (32)P and (3)H radioactivity, Pronase treatment had little effect on the macromolecular (32)P. Treatment of cells prelabeled with [(3)H]glucosamine and [(32)P]phosphate with insolubilized papain also revealed a parallel release of macromolecular glucosamine together with ribonuclease-susceptible macromolecular phosphate. Lactoperoxidase-mediated radioiodination of surface components in cells prelabeled with [(32)P]phosphate revealed electrophoretic comigration between the (125)I and the (32)P that are removed from the cells by mild proteolysis. Growth of the cells in Bt(2)cAMP-testosterone altered the kinetics of release and nature of the macromolecular (32)P liberated by proteolysis.
将预先用[（32）P]磷酸盐和[（3）H]亮氨酸标记的中国仓鼠卵巢细胞单层在细胞仍保持完全存活的条件下短暂暴露于1微克/毫升的胰蛋白酶，会导致大分子（32）P和（3）H的释放。虽然发现核糖核酸酶处理对（32）P和（3）H放射性均有显著影响，但链霉蛋白酶处理对大分子（32）P影响很小。用[（3）H]葡糖胺和[（32）P]磷酸盐预先标记的细胞用不溶性木瓜蛋白酶处理，也显示出大分子葡糖胺与对核糖核酸酶敏感的大分子磷酸盐平行释放。用[（32）P]磷酸盐预先标记的细胞中表面成分的乳过氧化物酶介导的放射性碘化显示，（125）I与通过温和蛋白酶解从细胞中去除的（32）P在电泳中共同迁移。细胞在Bt（2）cAMP-睾酮中生长改变了蛋白水解释放的大分子（32）P的释放动力学和性质。
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