Ihler G M, Glew R H, Schnure F W
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2663-6. doi: 10.1073/pnas.70.9.2663.
We demonstrated that beta-glucosidase and beta-galactosidase can be trapped inside erythrocytes by rapid hemolysis of the cell in the presence of these enzymes. Enzyme enters only during hemolysis, and optimum uptake occurs within 60 sec. There is no loss in cell number after hemolysis-induced enzyme uptake, and the ghosts have only a slightly increased mean cell volume. Smaller proteins enter more readily than larger proteins, although enzymes with a molecular weight of at least 180,000 can be readily entrapped by erythrocytes. This finding may provide a useful approach to the problem of enzyme replacement in certain diseases, including Gaucher's disease.
我们证明，在这些酶存在的情况下，通过细胞的快速溶血，β-葡萄糖苷酶和β-半乳糖苷酶可以被困在红细胞内。酶仅在溶血过程中进入，最佳摄取发生在60秒内。溶血诱导的酶摄取后细胞数量没有损失，且红细胞影的平均细胞体积仅略有增加。较小的蛋白质比较大的蛋白质更容易进入，尽管分子量至少为180,000的酶也可以很容易地被红细胞捕获。这一发现可能为某些疾病（包括戈谢病）的酶替代问题提供一种有用的方法。
