一种用于使红细胞负载酶和脂质的透析程序。

A dialysis procedure for loading erythrocytes with enzymes and lipids.

作者信息

Deloach J, Ihler G

出版信息

Biochim Biophys Acta. 1977 Jan 24;496(1):136-45. doi: 10.1016/0304-4165(77)90121-0.

Abstract

A dialysis procedure for hypotonic hemolysis has been developed in which erythrocytes can be loaded with water-soluble enzymes, detergent-solubilized enzymes (glucocerebrosidase) and detergent-dispersed glycolipid (glucocerebroside). The procedure allows approx. 40-50% of the added enzyme or glycolipid to be encapsulated. The final intracellular concentration of enzyme or glycolipid is about to the extracellular concentration. The loaded cells can be ingested by macrophage in vitro and the glucocerebroside partially degraded by lysosomal glucocerebrosidase. The use of this procedure for the investogation of Gaucher's disease is discussed.

摘要

已开发出一种用于低渗溶血的透析程序，通过该程序可使红细胞装载水溶性酶、去污剂增溶的酶（葡糖脑苷脂酶）和去污剂分散的糖脂（葡糖脑苷脂）。该程序可使大约40 - 50%添加的酶或糖脂被包封。酶或糖脂的最终细胞内浓度与细胞外浓度相近。装载后的细胞在体外可被巨噬细胞摄取，且葡糖脑苷脂可被溶酶体葡糖脑苷脂酶部分降解。讨论了该程序在戈谢病研究中的应用。